HLA-D REGION-ASSOCIATED DETERMINANTS SERVE AS TARGETS FOR HUMAN CELL-MEDIATED LYSIS
HLA-D REGION-ASSOCIATED DETERMINANTS SERVE AS TARGETS FOR HUMAN CELL-MEDIATED LYSIS
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DOI：
10.1084/jem.149.2.485
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发表时间：
1979-01-01
影响因子：
15.3
通讯作者：
STASTNY, P
中科院分区：
文献类型：
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作者：
FEIGHERY, C;STASTNY, P
通过体外培养来自选定供体的淋巴细胞与来自无关受试者的X-照射细胞来产生用于细胞介导裂解（CML）的效应细胞，所述无关受试者是HLA-D纯合的并且与HLA-A和HLA-B区域的抗原的应答者匹配。通过使用铬标记的单核细胞作为靶细胞，细胞毒性与HLA-D区抗原匹配的刺激细胞。这种明显针对HLA-D产物的CML反应可通过加入未标记的单核细胞或B [骨髓衍生]淋巴细胞来抑制。这些未标记的细胞必须与用于产生效应细胞群的刺激细胞匹配HLA-D。HLA-D的产物，可能是DR抗原，显然被细胞毒性淋巴细胞识别。
Effector cells for cell-mediated lysis (CML) were generated by in vitro culture of lymphocytes from selected donors with X-irradiated cells from unrelated subjects who were HLA-D homozygous and matched to the responders for the antigens of the HLA-A and HLA-B regions. By using Cr-labeled monocytes as target cells, cytotoxicity correlated with HLA-D region antigens matching those of the stimulating cells. Such CML reactions apparently directed at products of HLA-D, were inhibited by addition of unlabeled monocytes or B [bone marrow-derived] lymphocytes. These unlabeled cells had to be matched for HLA-D with the stimulating cells used to generate the effector populations. Products of HLA-D, perhaps the DR antigens, were apparently recognized by cytotoxic lymphocytes.