Identification, sequence and developmental expression of invertebrate flotillins from Drosophila melanogaster
Identification, sequence and developmental expression of invertebrate flotillins from Drosophila melanogaster
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DOI：
10.1016/s0378-1119(98)00064-x
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发表时间：
1998-04-14
期刊：
影响因子：
3.5
通讯作者：
Lisanti, MP
中科院分区：
文献类型：
--
作者：
Galbiati, F;Volonté, D;Lisanti, MP
小窝是囊状细胞器，代表质膜的一个亚室。Caveolins(Cav-L，-2和-3)和Flotillins[Flo-1和Flo-2.[也称为表皮表面抗原(ESAs)]是哺乳动物小窝相关的两个完整膜蛋白家族。尽管最近在无脊椎动物秀丽线虫中发现了小窝蛋白基因家族，但目前尚不清楚无脊椎动物中是否存在Flotillin同源物。本文报道了第一个无脊椎动物Flotillin的鉴定、基因序列和胚胎表达模式。Flodm与哺乳动物fltillin-1的亲缘关系最为密切。值得注意的是，无脊椎动物的Flodm蛋白的行为类似于哺乳动物的船队，在哺乳动物细胞中瞬时表达后，它被靶向于富含小凹的膜组分。对该基因在黑腹水母胚胎中的定位表明，该基因的表达主要局限于发育中的神经系统。这与我们之前观察到的哺乳动物Flotillin-1mRNA和蛋白在脑组织中大量表达的观察结果一致。有趣的是，该基因被定位在染色体52B1-B2区域。此外，我们发现至少有两个Flotillin相关基因在黑腹果蝇中表达，我们的研究结果为以果蝇为遗传系统剖析Flotillin在小窝和神经元发育中的作用提供了一个起点和系统的基础。(C)1998年爱思唯尔科学公司。
Caveolae are vesicular organelles that represent a sub-compartment of the plasma membrane. Caveolins (Cav-l, -2 and -3) and flotillins {FLO-1 and FLO-2. [also known as epidermal surface antigens (ESAs)]} are two families of mammalian caveolae-associated integral membrane proteins. Although a caveolin gene family has recently been described in the invertebrate Caenorhabditis elegans, it remains unknown as to whether flotillin homologues exist in invertebrates.Here, we report the identification, cDNA sequence and embryonic expression pattern of the first invertebrate flotillin, i.e. flotillin from Drosophila melanogaster (FLODm). FLODm is most closely related to mammalian flotillin-1. Remarkably, the invertebrate FLODm protein behaves like mammalian flotillins and is targeted to the caveolae-enriched membrane fraction after transient expression in mammalian cells. Localization of the FLODm message in D. melanogaster embryos reveals that expression of FLODm is confined primarily to the developing nervous system. This is consistent with our previous observation that mammalian flotillin-1 mRNA and protein is expressed abundantly in brain tissue. Interestingly, the FLODm gene is localized to chromosomal region 52 B1-B2. In addition, we find that at least two flotillin-related genes are expressed in D. melanogaster, Our current results provide a starting point and systematic basis for dissecting the role of flotillin in caveolae and neuronal development using Drosophila as a genetic system. (C) 1998 Elsevier Science B.V.