Effects of progressive desialylation on the rate of disappearance of immunoreactive HCG from plasma in rats.
Effects of progressive desialylation on the rate of disappearance of immunoreactive HCG from plasma in rats.
复制标题
渐进性去唾液酸化对大鼠血浆中免疫反应性 HCG 消失率的影响。
DOI：
10.1210/endo-89-1-11
复制
发表时间：
1971
期刊：
影响因子：
4.8
通讯作者：
G. Ashwell
中科院分区：
文献类型：
--
作者：
E. van Hall;J. Vaitukaitis;G. Ross;J. Hickman;G. Ashwell
一个特定的放射免疫测定法被用来测量血浆样品中的免疫反应性激素的浓度，在注射后的时间间隔完整的和去唾液酸化制剂的高度纯化的人绒毛膜促性腺激素（HCG）到假孕大鼠。HCG去唾液酸化至25%时，血浆半衰期降低50%（从25分钟降至11分钟）。在25-62%的范围内去除唾液酸导致血浆t1/2（从11至小于1分钟）更显著的降低，但去唾液酸大于62%没有额外的影响。这些观察结果表明，先前观察到的生物活性递减进行性去唾液酸化HCG的结果，部分是由于血浆中的激素半衰期的变化。（内分泌学89：11，1971）
A specific radioimmunoassay was used to measure concentrations of immunoreactive hormone in plasma samples taken at intervals following injection of intact and variably desialylated preparations of highly purified human chorionic gonadotropin (HCG) into pseudopregnant rats. Desialylation of HCG up to 25% decreased plasma half-life by 50% (from 25 to 11 min). Removal of sialic acid over the range of 25–62% resulted in a more profound reduction in plasma t½ (from 11 to less than 1 min) but desialylation greater than 62 % was without additional effect. The observations suggest that previously observed decrements in biologic activity following progressive desialylation of HCG result, in part, from changes in plasma half-life of the hormone. (Endocrinology 89: 11, 1971)