Distinct pattern of embryonic expression of the sea urchin CyI actin gene in Tripneustes gratilla.
Distinct pattern of embryonic expression of the sea urchin CyI actin gene in Tripneustes gratilla.
复制标题
Tripneustes gratilla 中海胆 CyI 肌动蛋白基因胚胎表达的独特模式。
DOI：
10.1006/dbio.1994.1239
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发表时间：
1994
影响因子：
2.7
通讯作者：
Humphreys,T
中科院分区：
文献类型：
--
作者：
Wang,AV;Angerer,LM;Dolecki,GJ;Lum,R;Wang,GV;Carlos,R;Angerer,RC;Humphreys,T
对Tripneustes gratillagenomic DNA和编码发育调节的胚胎信使RNA（称为Tg 616）的cDNA进行克隆和测序，揭示了编码肌动蛋白的基因，该基因与描述自Strongylocentrotus purpuratus的CyI肌动蛋白基因正向同源。（1）转录起始位点5′端150个核苷酸的高度保守序列，（2）蛋白质编码区95%的核苷酸序列同一性，在377个氨基酸残基中有375个氨基酸残基完全相同;（3）在信使RNA的3′非翻译区具有广泛的核苷酸序列同一性。因此，Tg 616被命名为TgCyI。原位杂交显示TgCyI在胚胎的各种细胞中的顺序激活。TgCyI mRNA在初级和次级间充质细胞准备进入囊胚腔时、在囊胚期的预期离口外胚层细胞中、在肠分化期间的肠细胞中以及在pluteus期的口外胚层中变得丰富。这种胚胎基因表达的模式比在S. purpuratus胚胎中观察到的发育上调基因的任何主要模式都要复杂，并且与SpCyI表达不同，SpCyI表达逐渐局限于肠道和口腔外胚层。
Cloning and sequencing ofTripneustes gratillagenomic DNA and cDNA encoding a developmentally regulated, embryonic messenger RNA, referred to as Tg616, revealed an actin-encoding gene orthologous to the CyI actin gene described fromStrongylocentrotus purpuratus.Tg616 and SpCyI share: (1) 150 nucleotides of highly conserved sequence 5′ of the transcription start site, (2) 95% nucleotide sequence identity in the protein encoding regions, which specify identical amino acid residues in 375 of 377 positions, and (3) extensive nucleotide sequence identity in the 3′ untranslated region of their messenger RNAs. Tg616 was therefore designated TgCyI.In situhybridization shows sequential activation of TgCyI in various cells of the embryo. TgCyI mRNA becomes abundant in primary and secondary mesenchyme cells as they prepare to enter the blastocoel, in prospective aboral ectoderm cells at blastula stage, in gut cells during gut differentiation, and in oral ectoderm at pluteus stage. This pattern of embryonic gene expression is more complex than any of the major patterns of developmentally upregulated genes observed inS. purpuratusembryos and is distinct from SpCyI expression which is progressively restricted to the gut and oral ectoderm.