Afferent fibres in cat ventral roots: electrophysiological and histological evidence.
Afferent fibres in cat ventral roots: electrophysiological and histological evidence.
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猫腹根的传入纤维：电生理学和组织学证据。
DOI：
10.1113/jphysiol.1986.sp016250
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Thiesson,D
中科院分区：
文献类型：
--
作者：
Azerad,J;Hunt,CC;Laporte,Y;Pollin,B;Thiesson,D
使用平均化技术的电生理实验，以及使用辣根过氧化物酶染色的解剖学实验，提供了猫腰骶腹根传入轴突的进一步证据。刺激靠近硬脑膜的伴行腹根后，从L7、S1或S2的背根丝记录到属于A三角洲或C群的慢传导动作电位。对前根近端的刺激不能引起这种反应。沿着中央切断的前根细丝的多个位置的记录显示出两种类型的反应：对周围神经刺激的长潜伏期动作电位，在所有记录位置都能看到，而在背根刺激后看不到。这些似乎是通过腹根进入脊髓的传入纤维；刺激背根后的动作电位，通常仅在最远端的腹根记录部位可见。其中一些也是通过刺激一些皮肤或肌肉神经来激活的。在适当的时间间隔，可以显示来自背根或周围神经的脉冲碰撞。这样的轴突似乎在腹根有一个周期性的过程。在逐渐靠近背根神经节的点切断脊神经，在神经节远端2 mm处取消大多数返型轴突的背根到腹根的连续性。将辣根过氧化物酶应用于切断的背根远端残端，可在伴行腹根的远端观察到酶标记的细小纤维。在前根的远端和神经节极部附近的脊神经中观察到轴突的U形转弯。
Electrophysiological experiments using averaging techniques, as well as anatomical experiments using horseradish peroxidase staining, have provided further evidence of afferent axons in lumbosacral ventral roots of cats. Recording from dorsal root filaments in L7, S1 or S2, following stimulation of the companion ventral root close to the dura, often shows action potentials of slow conduction velocity belonging to the A delta or C group. Stimulation applied to the proximal part of the ventral root failed to evoke such responses. Recording from multiple sites along a centrally cut ventral root filament shows responses of two types: action potentials of long latency to peripheral nerve stimulation which are seen at all recording locations and which are not seen following dorsal root stimulation. These appear to be afferent fibres which enter the cord via the ventral root; action potentials which follow dorsal root stimulation and which are usually seen only at the most distal ventral root recording site. Some of these were also activated by stimulation of some skin or muscle nerves. At appropriate intervals collision of impulses from dorsal root or peripheral nerve can be demonstrated. Such axons appear to have a recurrent course in the ventral root. Section of the spinal nerve at points progressively closer to the dorsal root ganglion abolishes the dorsal to ventral root continuity of most recurrent type axons at 2 mm distal to the ganglion. Following application of horseradish peroxidase to crushed ends of distal stumps of cut dorsal roots, thin fibres marked by the enzyme are observed in the distal part of companion ventral roots. U‐turns of axons have been observed in the distal part of ventral roots and in the spinal nerve near the pole of the ganglion.