Binding of Sperm to the Zona Pellucida Mediated by Sperm Carbohydrate-Binding Proteins is not Species-Specific in Vitro between Pigs and Cattle.
Binding of Sperm to the Zona Pellucida Mediated by Sperm Carbohydrate-Binding Proteins is not Species-Specific in Vitro between Pigs and Cattle.
复制标题
DOI：
10.3390/biom3010085
复制
发表时间：
2013-01-25
期刊：
影响因子：
5.5
通讯作者：
Yonezawa N
中科院分区：
文献类型：
--
作者：
Takahashi K;Kikuchi K;Uchida Y;Kanai-Kitayama S;Suzuki R;Sato R;Toma K;Geshi M;Akagi S;Nakano M;Yonezawa N
在哺乳动物受精过程中，碳水化合物是配子物种选择性相互作用的候选物质。在这项研究中，我们试图阐明糖残留物在猪和牛的物种选择性、精子-卵母细胞相互作用中的作用。顶体完整的猪和牛精子分别与β-Gal和α-Man残基显示出最强的结合亲和力。顶体反应后，猪精子特异性由β-Gal转变为α-Man，而牛精子特异性不变。顶体完整的精子和顶体反应的精子经胰酶消化后结合能力降低，说明结合碳水化合物的成分为蛋白质。虽然未成熟的卵母细胞优先结合同种异体精子，但体外成熟的卵母细胞结合的同种和异种精子数量相似。凝集素染色显示成熟过程中α-MAN残基聚集在猪卵透明带外表面。在这两个物种中，去透明带的成熟卵母细胞优先结合同种精子而不是异种精子。透明带和去透明带卵母细胞的凝集素染色模式分别与顶体完整和顶体反应的精子的碳水化合物结合特性一致，支持碳水化合物参与猪和牛的配子识别。这些结果还表明，猪和牛的精子-透明带和精子-卵膜结合并不是严格意义上的物种特异性，进一步表明精子穿透透明带和/或与卵膜融合可能是猪和牛之间的物种特异性。
Carbohydrates are candidates for the basis of species-selective interaction of gametes during mammalian fertilization. In this study, we sought to clarify the roles of sugar residues in the species-selective, sperm–oocyte interaction in pigs and cattle. Acrosome-intact porcine and bovine sperm exhibited their strongest binding affinities for β-Gal and α-Man residues, respectively. Porcine-sperm specificity changed from β-Gal to α-Man after the acrosome reaction, while bovine-sperm specificity did not. Binding of acrosome-intact and acrosome-reacted sperm decreased after trypsinization, indicating that the carbohydrate-binding components are proteins. While immature oocytes bound homologous sperm preferentially to heterologous sperm, oocytes matured in vitro bound similar numbers of homologous and heterologous sperm. Lectin staining revealed the aggregation of α-Man residues on the outer surface of the porcine zona during maturation. In both species, zona-free, mature oocytes bound homologous sperm preferentially to heterologous sperm. The lectin-staining patterns of the zona pellucida and zona-free oocytes coincided with the carbohydrate-binding specificities of acrosome-intact and acrosome-reacted sperm, respectively, supporting the involvement of carbohydrates in gamete recognition in pigs and cattle. These results also indicate that sperm-zona pellucida and sperm–oolemma bindings are not strictly species-specific in pigs and cattle, and further suggest that sperm penetration into the zona and/or fusion with oolemma may be species-specific between pigs and cattle.