Diseases of Aquatic Organisms Dis Aquat Org
Diseases of Aquatic Organisms Dis Aquat Org
复制标题
DOI：
--
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
三种鲑性鱼立克次体分离物对银鲑的相对毒力摘要：鲑性鱼立克次体在智利首次被认为是导致圈养银鲑死亡的原因。自1989年首次分离出这种细胞内革兰氏阴性细菌以来，世界上多个地区都发现了类似的微生物，但相关的疫情报告没有智利发生的疫情那么严重。为了确定这是否是由于鲑鱼疫霉分离株之间毒力的差异，我们进行了一项实验，比较了来自智利、不列颠哥伦比亚省、加拿大和挪威的分离株（分别为LF-89、ATL-4-91和NOR-92）。对于每种分离物，用3种浓度的细菌中的每一种腹膜内注射30条银鲑的3个重复。阴性对照鱼注射MEM-10。注射后41 d每天收集死亡率。在注射3种分离株的鱼中观察到鱼立克次体病，对于每种分离株，累积死亡率与给予的细菌细胞浓度直接相关。LF-89分离株的毒力最强，用105注射的3次重复中的损失达到97%。在104.0 TCIDS 0的重复中，91%，在103.0 TCIDS 0的重复中，57%。ATL-4-91分离株在用IO '注射的3个重复中引起92%的损失。TCIDS 0，104.0 TCIDS 0组为76%，103. 0 TCIDS 0组为32%。TCIDSo。NOR-92分离株毒性最低，在用104. TCIDSO。在注射后41天，注射1036 TCIDSO NOR-92的鱼中有6%死亡。注射102.6TCID，NOR-92的鱼的死亡率仅为2%，与阴性对照组相同。由于注射最高浓度（104 TCIDS 0）NOR-92的组在41天时仍出现死亡，因此将其再保持46天。注射后87 d，该组的累积死亡率已达70%。这些菌株之间的毒力差异具有统计学显著性（p < 0.0001），对于受影响鱼类种群的管理非常重要。
Relative virulence of three isolates of Piscirickettsia salmonis for coho salmon Oncorhynchus kisutch ABSTRACT: Piscinckettsia salmonis was first recognized as the cause of mortality among pen-reared coho salmon Oncorhynchus kisutch in Chile. Since the initial isolation of this intracellular Gram-negative bacterium in 1989, similar organisms have been described from several areas of the world, but the associated outbreaks were not reported to be as serious as those that occurred in Chile. To determine if this was due to differences in virulence among isolates of P. salmonis, we conducted an experiment comparing isolates from Chile, British Columbia, Canada, and Norway (LF-89, ATL-4-91 and NOR-92, respectively). For each of the isolates, 3 replicates of 30 coho salmon were injected intraperitoneally with each of 3 concentrations of the bacterium. Negative control fish were injected with MEM-10. Mor-talities were collected d d y for 41 d post-injection. Piscirickettsiosis was observed in fish injected with each of the 3 isolates, and for each isolate, cumulative mortality was directly related to the concentration of bacterial cells administered. The LF-89 isolate was the most virulent, with losses reaching 97 % in the 3 replicates injected with 105.' TCIDS0, 91 % in the replicates injected with 104 O TCIDSO, and 57 % in the fish injected with 103.0 TCIDS0. The ATL-4-91 isolate caused losses of 92 % in the 3 replicates injected with IO'.~ TCIDS0, 76% in the fish injected with 104.0 TCIDSO, and 32% in those injected with 103.' TCIDSo. The NOR-92 isolate was the least virulent, causing 41 % mortality in the replicates injected with 104.= TCIDSO. At 41 d post-injection, 6 % of the fish injected with 1036 TCIDSO NOR-92 had died. Mortality was only 2 % in the fish injected with 102.6 TCID,, NOR-92, which was the same as the negative control group. Because the group injected with the highest concentration (104 TCIDS0) of NOR-92 was still experiencing mortality at 41 d, it was held for an additional 46 d. At 87 d post-injection , the cumulative mortality in this group had reached 70%. These differences in virulence among the isolates were statistically significant (p < 0.0001), and are important for the management of affected stocks of fish.