A Single-Transaminase-Catalyzed Biocatalytic Cascade for Efficient Asymmetric Synthesis ofl-Phosphinothricin
A Single-Transaminase-Catalyzed Biocatalytic Cascade for Efficient Asymmetric Synthesis ofl-Phosphinothricin
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DOI：
10.1002/cbic.202000488
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发表时间：
2020-09-28
期刊：
影响因子：
3.2
通讯作者：
Zheng, Yu-Guo
中科院分区：
文献类型：
--
作者：
Cheng, Feng;Li, Ju-Mou;Zheng, Yu-Guo
采用所需要的生物催化剂ATA-117-Rd11，建立了单转氨酶催化的生物级联反应，该生物催化剂对2-氧-4-[（羟基）（甲基）膦酰]丁酸（PPO）和α -酮戊二酸具有高活性，对丙酮酸具有低活性。该级联成功地促进了一个高度不对称的胺化反应，合成了高转化率（> 95%）和> 99% ee的-膦-丙氨酸（l-PPT）。在放大实验中，使用10公斤预冷冻的ene。以ATA-117-Rd11为催化剂的coliccells，在24小时后，80 kg PPO转化为约70 kg - ppt，并具有较高的评价（> 99%）。
A single-transaminase-catalyzed biocatalytic cascade was developed by employing the desired biocatalyst, ATA-117-Rd11, that showed high activity toward 2-oxo-4-[(hydroxy)(methyl)phosphinoyl] butyric acid (PPO) and alpha-ketoglutarate, and low activity against pyruvate. The cascade successfully promotes a highly asymmetric amination reaction for the synthesis ofl-phosphinothricin (l-PPT) with high conversion (>95 %) and>99 %ee. In a scale-up experiment, using 10 kg pre-frozenE. colicells harboring ATA-117-Rd11 as catalyst, 80 kg PPO was converted to approximate to 70 kgl-PPT after 24 hours with a higheevalue (>99 %).