Effect of chronic Taura syndrome virus infection on salinity tolerance of Litopenaeus vannamei.
Effect of chronic Taura syndrome virus infection on salinity tolerance of Litopenaeus vannamei.
复制标题
慢性陶拉综合征病毒感染对凡纳滨对虾耐盐性的影响。
DOI：
10.3354/dao065075
复制
发表时间：
2005
影响因子：
1.4
通讯作者：
M. Soto
中科院分区：
文献类型：
--
作者：
J. Lotz;Lesber Salazar Anton;M. Soto
陶拉综合征病毒(TSV)是影响世界范围内养殖对虾的最重要的对虾病毒之一。在经历了死亡率上升的急性期后，受感染的虾进入慢性期，在此期间，虾似乎恢复了正常的行为，没有明显的感染迹象。这项研究旨在确定长期感染TSV的凡纳滨对虾是否受到感染的影响。具体地说，我们调查了长期感染的虾是否能像未感染的虾一样耐受盐度的下降。这项研究包括3个试验，比较了盐度从24ppt下降到18ppt到0ppt后，未感染和长期感染TSV的南美白纹伊蚊的存活率。Logistic回归分析发现TSV感染对慢性感染的对虾存活率有显著影响(p<；0.05)。盐度从24ppt下降到3ppt和6ppt，导致了统计学上不同的存活率(p<；0.05)。盐度下降大于6ppt或低于3ppt的组之间的存活率相似。对虾50%死亡的盐度(LC_(50))未感染组为3.06 ppt，慢性感染组为6.65 ppt。此外，对垂死或最近死亡的慢性感染虾进行的组织病理学分析显示，没有迹象表明该疾病已恢复到急性期。这些结果表明，长期感染的虾不能像未感染的虾那样耐受盐度下降。
Taura syndrome virus (TSV) is one of the most important shrimp viruses affecting farmed shrimp worldwide. After an acute phase during which the likelihood of mortality is elevated, infected shrimp enter a chronic phase during which shrimp appear to resume normal behavior and display no gross signs of infection. This study was designed to determine if chronically TSV-infected shrimp Litopenaeus vannamei are compromised by the infection. Specifically we investigated whether chronically infected shrimp could tolerate a drop in salinity as strongly as uninfected shrimp. The study consisted of 3 trials that compared survival of uninfected and chronically TSV-infected L. vannamei after drops in salinity from 24 ppt to salinities varying from 18 to 0 ppt. Logistic regression detected a significant effect of TSV infection on survival of chronically infected shrimp (p < 0.05). Salinity drops from 24 ppt to 3 and 6 ppt resulted in statistically different survivals (p < 0.05). Survival rates were similar among groups for salinity drops to greater than 6 ppt or less than 3 ppt. Salinities at which 50% of the shrimp died (LC50) were 3.06 ppt for the uninfected and 6.65 ppt for the chronically infected groups. Moreover, histopathological analysis of chronically infected shrimp that were moribund or recently dead showed no signs of having reverted to the acute stage of the disease. These results suggest that chronically infected shrimp are not able to tolerate a salinity drop as strongly as uninfected shrimp.