A cascading reaction sequence involving ligand-directed azaelectrocyclization and autooxidation-induced fluorescence recovery enables visualization of target proteins on the surfaces of live cells.
A cascading reaction sequence involving ligand-directed azaelectrocyclization and autooxidation-induced fluorescence recovery enables visualization of target proteins on the surfaces of live cells.
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涉及配体引导的氮杂电环化和自氧化诱导的荧光恢复的级联反应序列能够实现活细胞表面目标蛋白的可视化。
DOI：
10.1039/c3ob42267d
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Fukase K.
中科院分区：
文献类型：
--
作者：
Tanaka;K.;Kitadani;M.;Tsutsui;A.;Pradipta;AR.;Imamaki;R.;Kitazume;S.;Taniguchi;N.;Fukase K.
有效地合成了一种通用探针，该探针旨在诱导目标蛋白上的级联反应序列。级联反应序列包括(I)配体引导的与赖氨酸的氮杂电环反应和(Ii)自氧化诱导标记蛋白释放荧光猝灭剂。该探针被连接到一个环状RGDyK肽上，使整合素αVβ3能够选择性地显示在活细胞表面。
A general probe designed to induce a cascading sequence of reactions on a target protein was efficiently synthesized. The cascading reaction sequence involved (i) ligand-directed azaelectrocyclization with lysine and (ii) the autooxidation-induced release of a fluorescence quencher from the labeled protein. The probe was linked to a cyclic RGDyK peptide to enable the selective visualization of integrin αVβ3 on the surfaces of live cells.