High-efficiency yeast artificial chromosome fragmentation vectors.
High-efficiency yeast artificial chromosome fragmentation vectors.
复制标题
高效酵母人工染色体断裂载体。
DOI：
10.1016/0378-1119(91)90576-w
复制
发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
Reeves,RH
中科院分区：
文献类型：
--
作者：
Pavan,WJ;Hieter,P;Sears,D;Burkhoff,A;Reeves,RH
染色体片段化载体（CFV）用于产生克隆为酵母人工染色体（YAC）的人DNA的大片段的缺失衍生物。CFV靶向端粒序列插入YAC通过同源重组与重复元件。该事件导致整合位点远端的所有YAC序列的丢失。一个新的CFV系列已经开发出来。这些载体将片段化靶向于人和LINE人重复DNA元件。用新载体回收缺失衍生物的效率比用先前描述的载体高10至20倍。
Chromosome fragmentation vectors (CFVs) are used to create deletion derivatives of large fragments of human DNA cloned as yeast artificial chromosomes (YACs). CFVs target insertion of a telomere sequence into the YAC via homologous recombination withAlurepetitive elements. This event results in the loss of allYACsequences distal to the site of integration. A new series of CFVs has been developed. These vectors target fragmentation to bothAluandLINEhuman repetitive DNA elements. Recovery of deletion derivatives is ten- to 20-fold more efficient with the new vectors than with those described previously.