Isolation and characterization of the Rhodococcus opacus thiostrepton-inducible genes tipAL and tipAS: application for recombinant protein expression in Rhodococcus.
Isolation and characterization of the Rhodococcus opacus thiostrepton-inducible genes tipAL and tipAS: application for recombinant protein expression in Rhodococcus.
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DOI：
10.1111/j.1574-6968.2004.tb09675.x
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发表时间：
2004-08
影响因子：
2.1
通讯作者：
Li Dong;N. Nakashima;N. Tamura;T. Tamura
中科院分区：
文献类型：
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作者：
Li Dong;N. Nakashima;N. Tamura;T. Tamura
我们克隆了不透明红球菌（菌株DSM 44193）tipA基因，其编码两种翻译产物TipAL和TipAS。该基因产物与链霉菌同源。TipAL和TipAS蛋白。tipA启动子具有很高的活性，TipAS蛋白主要在R.当在培养基中存在转录诱导剂硫链丝菌素时，我们发现，硫链丝菌肽也诱导表达的内源性TipA家族蛋白红串红球菌（菌株JCM 3201）。最小的tipA启动子区域被定义（57 bp）和保守的核苷酸序列的推定的TipAL蛋白结合位点（TipA-box）被确定在该区域。推测tipA基因被转录成无前导的mRNA。我们成功地将tipA启动子应用于重组蛋白在R.红细胞
We cloned the Rhodococcus opacus (strain DSM 44193) tipA gene, which encodes two translation products, TipAL and TipAS. The gene products are homologous to the Streptomyces spp. TipAL and TipAS proteins, respectively. The tipA promoter is highly active and TipAS protein is predominantly accumulated in R. opacus cells when the inducer of transcription, thiostrepton, was presented in culture medium. We found that thiostrepton is also induced the expression of an endogenous TipA-family protein in Rhodococcus erythropolis (strain JCM3201). The minimal tipA promoter region was defined (57 bp) and the conserved nucleotide sequence of the putative TipAL protein binding site (TipA-box) was identified in that region. The tipA gene is presumed to be transcribed into a leaderless mRNA. We applied the tipA promoter successfully for recombinant protein expression in R. erythropolis cells.