Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study
Nonribosomal peptides for iron acquisition: pyochelin biosynthesis as a case study
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DOI：
10.1016/j.sbi.2018.01.015
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发表时间：
2018-12-01
影响因子：
6.8
通讯作者：
Lamb, Audrey L.
中科院分区：
文献类型：
--
作者：
Ronnebaum, Trey A.;Lamb, Audrey L.
微生物合成小的铁螯合分子，称为铁载体，从环境中获取铁。铁载体产生的一种方式是通过非核糖体肽合成酶（NRPS）。由NRPS酶产生的生物活性肽具有独特的化学特征，这些化学特征通过附属结构域和剪裁结构域或蛋白质并入。本文第一部分综述了NRPS结构生物学的最新进展。第二部分使用绿脓菌螯铁蛋白，从铜绿假单胞菌的铁载体的生物合成，作为一个案例研究，以检查酶的方法产生观察到的NRPS衍生的天然产物的多样性。
Microbes synthesize small, iron-chelating molecules known as siderophores to acquire iron from the environment. One way siderophores are generated is by nonribosomal peptide synthetases (NRPSs). The bioactive peptides generated by NRPS enzymes have unique chemical features, which are incorporated by accessory and tailoring domains or proteins. The first part of this review summarizes recent progress in NRPS structural biology. The second part uses the biosynthesis of pyochelin, a siderophore from Pseudomonas aeruginosa, as a case study to examine enzymatic methods for generating the observed diversity in NRPS-derived natural products.