Separation techniques: Chromatography.
Separation techniques: Chromatography.
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DOI：
10.14744/nci.2016.32757
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发表时间：
2016
影响因子：
1
通讯作者：
Coskun O
中科院分区：
文献类型：
--
作者：
Coskun O
色谱法是一种重要的生物物理技术，可以分离、鉴定和纯化混合物的成分，用于定性和定量分析。蛋白质可以根据诸如大小和形状、总电荷、表面上存在的疏水性基团以及与固定相的结合能力等特征进行纯化。基于分子特性和相互作用类型的四种分离技术分别采用离子交换、表面吸附、分割和尺寸排斥机制。其他色谱技术是基于固定床，包括柱，薄层和纸色谱。柱层析是蛋白质纯化最常用的方法之一。
Chromatography is an important biophysical technique that enables the separation, identification, and purification of the components of a mixture for qualitative and quantitative analysis. Proteins can be purified based on characteristics such as size and shape, total charge, hydrophobic groups present on the surface, and binding capacity with the stationary phase. Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography. Column chromatography is one of the most common methods of protein purification.