The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst
The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst
复制标题
DOI：
10.1017/s0031182006000400
复制
发表时间：
2006-09-01
期刊：
影响因子：
2.4
通讯作者：
Mortara, R. A.
中科院分区：
文献类型：
--
作者：
Bahia, D.;Avelar, L. G. A.;Mortara, R. A.
用鬼臼毒素-罗丹明标记曼氏血吸虫卵、毛虫和初生孢子囊，以显示丝状肌动蛋白结构。通过共聚焦荧光显微镜对这些形式的分析发现，先前明确的环状和纵向肌层的存在。除了这些维持这些寄生虫形式并为其提供运动的肌层外，我们还在这三个连续的发育阶段发现了以前未被识别的富含肌动蛋白的管状结构。光镜切片可见3种形态的肌层下均可见4条富含肌动蛋白的小管。小管成对出现，与寄生虫的长度成对，位于四肢。通过使用抗火焰细胞特异性抗体，我们证实了小管与火焰细胞共定位，并确定了小管核心充满了微管。肌球蛋白在这些小管中的额外存在强烈地表明它们是收缩结构。
Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular layers that sustain and provide motility to these parasite forms, we found in these 3 consecutive developmental stages of the parasite previously unidentified actin-rich tubular structures. In the 3 forms, 4 actin-rich tubules could be observed by optical sectioning underneath the well-developed muscle layers. The tubules appear in pairs, transversal to the length of the parasite, and located towards the extremities. By using an anti-flame cell specific antibody we confirmed that the tubules co-localize with flame cells and also determined that the tubule core is filled with microtubules. The additional presence of myosin in these tubules strongly suggests that they are contractile structures.