Dietary phytanic acid-induced changes in tissue fatty acid profiles in mice
Dietary phytanic acid-induced changes in tissue fatty acid profiles in mice
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膳食植烷酸引起小鼠组织脂肪酸谱的变化
DOI：
10.1017/s0022029920001089
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发表时间：
2020
影响因子：
2.1
通讯作者：
Kawahara Satoshi
中科院分区：
文献类型：
--
作者：
Nakanishi Tomonori;Kagamizono Kazuhiro;Yokoyama Sayaka;Suzuki Ryoji;Sakakibara Hiroyuki;Sugamoto Kazuhiro;Erickson Laurie;Kawahara Satoshi
本研究通讯的目的是研究植烷酸（PA）在小鼠体内组织中的蓄积和组织脂肪酸谱的任何变化。以前的体外研究已经证明，PA是一种牛奶成分，有可能对脂质和葡萄糖代谢产生有益影响，对神经元细胞产生有害影响。然而，关于其体内作用的信息有限。在这项研究中，给小鼠喂食含有0.00或0.05% 3RS，7 R，11 R-PA的饲料，3RS，7 R，11 R-PA是牛奶和人体中发现的异构体。4周后，收获脂肪组织、肝脏和脑，并通过气相色谱分析测定其脂肪酸谱。结果表明，PA及其代谢产物降植烷酸在脂肪组织中积累，PA降低了肝脏中棕榈酸等饱和脂肪酸的组成，增加了亚油酸和二十二碳六烯酸等多不饱和脂肪酸的组成。然而，膳食PA既不积累，也没有对大脑中的脂肪酸谱产生很大影响。这些结果表明，膳食PA可在脂肪组织和肝脏中发挥其生物活性，而大脑受膳食PA的影响相对较小。这些数据为了解PA的体内生理作用提供了基础。
The aims of this research communication were to investigate the in vivo tissue accumulation of phytanic acid (PA) and any changes in the tissue fatty acid profiles in mice. Previous in vitro studies have demonstrated that PA is a milk component with the potential to cause both beneficial effects on lipid and glucose metabolism and detrimental effects on neuronal cells. However, there is limited information about its in vivo actions. In this study, mice were fed diets containing either 0.00 or 0.05% 3RS, 7R, 11R-PA, which is the isomer found in milk and the human body. After 4 weeks, adipose tissue, liver and brain were harvested and their fatty acid profiles were determined by gas chromatographic analysis. The results showed that PA and its metabolite pristanic acid accumulated in the adipose tissue of PA-fed mice, and that dietary PA decreased the hepatic compositions of several saturated fatty acids such as palmitic acid while increasing the compositions of polyunsaturated fatty acids including linoleic acid and docosahexaenoic acid. However, dietary PA neither accumulated nor had a high impact on the fatty acid profile in the brain. These results suggested that dietary PA could exert its biological activities in adipose tissue and liver, although the brain is relatively less affected by dietary PA. These data provide a basis for understanding the in vivo physiological actions of PA.