Detection of RNA from a Novel West Nile-like Virus and High Prevalence of an Insect-specific Flavivirus in Mosquitoes in the Yucatan Peninsula of Mexico
Detection of RNA from a Novel West Nile-like Virus and High Prevalence of an Insect-specific Flavivirus in Mosquitoes in the Yucatan Peninsula of Mexico
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DOI：
10.4269/ajtmh.2009.80.85
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发表时间：
2009-01-01
影响因子：
3.3
通讯作者：
Blitvich, Bradley J.
中科院分区：
文献类型：
--
作者：
Farfan-Ale, Jose A.;Lorono-Pino, Maria A.;Blitvich, Bradley J.
作为我们对墨西哥尤卡坦半岛西尼罗河病毒持续监测工作的一部分。2007年1月至12月收集了96.687只蚊子，通过哺乳动物细胞中的病毒分离进行了检测。三个蚊池引起细胞病变效应。两个分离株为正布尼亚病毒（Cache Valley病毒和开里病毒），第三个感染因子的身份尚未确定。蚊子的一个子集也进行了测试逆转录聚合酶链反应（RT-PCR）使用西尼罗河病毒，黄病毒，甲病毒，和正布尼亚病毒特异性引物。共分析了210个样本池中的7，009只致倦库蚊。在146个（70%）样本池中检出黄病毒RNA。并对所有PCR产物进行测序。其中一个PCR产物的核苷酸序列与其他几种黄病毒的同源区最密切相关（71-73%同一性）。包括WNV、圣路易斯脑炎病毒和伊尔赫斯病毒。这些数据表明墨西哥存在一种新型黄病毒（暂命名为T 'Ho病毒）。其他145个PCR产物对应于库蚊黄病毒，这是2003年在日本首次分离的昆虫特异性黄病毒。库蚊黄病毒从大约四分之一的测试匀浆中分离出。确定了一个分离株的基因组序列。令人惊讶的是，在5'非翻译区的远端鉴定出了异质序列。
As part of our ongoing surveillance efforts for West Nile virus (WNV) ill the Yucatan Peninsula of Mexico. 96.687 mosquitoes collected from January through December 2007 were assayed by virus isolation in mammalian cells. Three mosquito pools caused cytopathic effect. Two isolates were orthobunyaviruses (Cache Valley virus and Kairi virus) and the identity of the third infectious agent was not determined. A subset of mosquitoes was also tested by reverse transcription-polymerase chain reaction (RT-PCR) using WNV-,flavivirus-, alphavirus-, and orthobunyavirus-specific primers. A total of 7,009 Cidex quinquefasciatus in 210 pools were analyzed. Flavivirus RNA Was detected in 146 (70%) pools. and all PCR products Were sequenced. The nucleotide sequence of one PCR product was most closely related (71-73% identity) with homologous regions of several other flaviviruses. including WNV, St. Louis encephalitis virus, and Ilheus virus. These data suggest that a novel flavivirus (tentatively named T'Ho virus) is present in Mexico. The other 145 PCR products correspond to Culex flavivirus, an insect-specific flavivirus first isolated in Japan in 2003. Culex flavivirus Was isolated in mosquito cells from approximately one in four homogenates tested. The genomic sequence of one isolate was determined. Surprisingly, heterogeneous sequences were identified at the distal end of the 5' untranslated region.