Injectable pulverized electrospun poly(lactic-co-glycolic acid) fibers improve human adipose tissue engraftment and volume retention.
Injectable pulverized electrospun poly(lactic-co-glycolic acid) fibers improve human adipose tissue engraftment and volume retention.
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可注射的粉末状电纺聚（乳酸-乙醇酸）纤维可改善人体脂肪组织的植入和体积保留。
DOI：
10.1002/jbm.a.37581
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Houser,Rober
中科院分区：
文献类型：
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作者：
Das,Devleena;Lawrence,WilliamR;Diaz-Starokozheva,Ludmila;Salazar-Puerta,AnaI;Ott,Neil;Goebel,ErinR;Damughatla,Abhishek;Vidal,Pablo;Gallentine,Summer;Moore,JordanT;Kayuha,Douglas;Mendonca,NataliaC;Albert,JaredB;Houser,Rober
自体脂肪组织通常用于软组织重建目的的组织移植，因为它在人体内相对丰富，并且易于使用吸脂方法获取。这导致了自体脂肪移植手术的采用，该手术允许注射脂肪组织作为“填充物”，以纠正软组织的美容缺陷和畸形。然而，此类方法的临床使用存在一些局限性，包括吸收率高和细胞存活率差，导致移植物体积保留率低和结果不一致。在这里，我们描述了磨碎电纺聚（乳酸-乙醇酸）（PLGA）纤维的一种新应用，该纤维可以与脂肪组织共同注射以改善植入结果。这些 PLGA 纤维在体外对脂肪细胞的活力没有显着的负面影响，并且在体内不会引起长期的促炎症反应。此外，与单独注射脂肪组织相比，将人类脂肪组织与粉末状电纺 PLGA 纤维共同输送可显着改善再灌注、血管分布和移植物体积保留。总而言之，使用磨碎的电纺纤维来增强自体脂肪移植技术代表了一种改进此类方法缺点的新方法。
Autologous adipose tissue is commonly used for tissue engraftment for the purposes of soft tissue reconstruction due to its relative abundance in the human body and ease of acquisition using liposuction methods. This has led to the adoption of autologous adipose engraftment procedures that allow for the injection of adipose tissues to be used as a “filler” for correcting cosmetic defects and deformities in soft tissues. However, the clinical use of such methods has several limitations, including high resorption rates and poor cell survivability, which lead to low graft volume retention and inconsistent outcomes. Here, we describe a novel application of milled electrospun poly(lactic‐co‐glycolic acid) (PLGA) fibers, which can be co‐injected with adipose tissue to improve engraftment outcomes. These PLGA fibers had no significant negative impact on the viability of adipocytes in vitro and did not elicit long‐term proinflammatory responses in vivo. Furthermore, co‐delivery of human adipose tissue with pulverized electrospun PLGA fibers led to significant improvements in reperfusion, vascularity, and retention of graft volume compared to injections of adipose tissue alone. Taken together, the use of milled electrospun fibers to enhance autologous adipose engraftment techniques represents a novel approach for improving upon the shortcomings of such methods.