IDENTIFICATION OF A HIGH-MOLECULAR-WEIGHT (GREATER-THAN 400 000) PROTEIN IN HYALINE CARTILAGE
IDENTIFICATION OF A HIGH-MOLECULAR-WEIGHT (GREATER-THAN 400 000) PROTEIN IN HYALINE CARTILAGE
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DOI：
10.1016/0304-4165(84)90370-2
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
BRANDT, KD
中科院分区：
文献类型：
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作者：
FIFE, RS;BRANDT, KD
A high-MW (> 400,000) non-collagenous protein was identified in normal articular cartilage from several mammalian species and in bovine tracheal cartilage. This protein is reduced by 2-mercaptoethanol to subunits with a MW of 116,000, which appear to constitute .apprx. 2-4% of the total protein detectable by the Lowry assay in 4 M guanidinium chloride extracts of normal bovine and canine articular cartilage. Antiserum to the 116 kDa [kilodalton] subunit protein from bovine articular cartilage cross-reacts with the intact and subunit proteins from bovine trachea and from normal canine, porcine and human articular cartilage. This protein is not found in non-cartilagenous tissues, suggesting that it is a cartilage-specific protein. The > 400 kDa protein and its subunit are ubiquitous and quantitatively significant proteins in hyaline cartilage.
