The junctional epithelium around murine teeth differs from gingival epithelium in its basement membrane composition
The junctional epithelium around murine teeth differs from gingival epithelium in its basement membrane composition
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DOI：
10.1177/00220345010800121401
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发表时间：
2001-12-01
影响因子：
7.6
通讯作者：
Hormia, M
中科院分区：
文献类型：
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作者：
Oksanen, J;Sorokin, LM;Hormia, M
目前尚不清楚牙龈基底膜（BM）的组成是否反映了上皮分化模式。我们已经调查了表达层粘连蛋白亚型和相关的BM组件在小鼠牙上皮连接使用免疫荧光显微镜。我们的研究结果表明，层粘连蛋白5/6/7/10/11的链在外牙龈上皮的BM中表达。结合上皮（JE）和结缔组织之间的外部BM与牙龈BM的不同之处在于缺乏层粘连蛋白-7和-11链。JE和牙齿之间的内部基底层（IBL）仅含有层粘连蛋白-5。胶原蛋白链α 1，2（IV）和巢蛋白-1存在于除IBL外的其他BM中。因此，牙-上皮连接具有独特的BM组成，表明上皮细胞能够以极化方式分泌两种细胞外基质。非自聚合层粘连蛋白-5的排他性表达表明IBL不是定义的BM，而是缺乏网络结构的简单细胞外基质。
It is not known whether epithelial differentiation patterns are reflected in the composition of gingival basement membranes (BMs). We have investigated the expression of laminin isoforms and associated BM components in the murine dento-epithelial junction by using immunofluorescence microscopy. Our results show that chains of laminins 5/6/7/10/11 are expressed in the BM of outer gingival epithelium. The external BM between junctional epithelium (JE) and connective tissue differs from gingival BM by lacking laminin-7 and -11 chains. The internal basal lamina (IBL) between JE and tooth contains only laminin-5. Collagen chains alpha1,2(IV) and nidogen-1 are present in other BMs except the IBL. The dento-epithelial junction thus has a unique BM composition, suggesting that epithelial cells are able to secrete two extracellular matrices in a polarized manner. The exclusive expression of the non-self-polymerizing laminin-5 indicates that the IBL is not a BM by definition, but rather a simple extracellular matrix lacking network structure.