In situ Analysis of Coral Recruits Using Fluorescence Imaging
In situ Analysis of Coral Recruits Using Fluorescence Imaging
复制标题
使用荧光成像对珊瑚新成员进行原位分析
DOI：
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发表时间：
2017
影响因子：
3.7
通讯作者：
T. Treibitz
中科院分区：
文献类型：
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作者：
Adi Zweifler;D. Akkaynak;T. Mass;T. Treibitz
补充是一个基本的过程，在acquirements珊瑚种群动态以及珊瑚礁群落结构。到目前为止，珊瑚补充成功率很难量化，因为调查方法是劳动密集型的，需要人工解释。因此，它们容易出现人为错误，重复性低-我们的目标是在这项研究中弥合这一差距。由于珊瑚和它们的共生藻类都含有荧光色素（叶绿素和荧光蛋白），我们使用了非侵入性荧光成像系统（FluorIS），并开发了一种方法来获取白天的荧光照片，并识别其中的珊瑚新兵。我们测试了我们的方法，监测20个随机样方在两个地点在以色列的亚喀巴湾。研究人员每月对这些样方进行一次调查，为期八个月，以追踪新珊瑚的定居、死亡和存活率。我们演示白天成像使用我们的方法和识别珊瑚新兵小至1毫米直径，在20 × 20厘米的样方。我们的研究结果表明，这种摄影方法减少了测量误差，提高了精度。调查显示，平均每个月有约2个新的珊瑚补充定居点（<2cm），其中83%的珊瑚在第一个月存活。我们的研究表明，在调查期间，在亚喀巴海湾珊瑚种群相对稳定。在世界珊瑚礁因地方和全球压力而减少之际，利用低成本、易于使用的摄影设备在白天对新珊瑚礁进行调查的能力有可能大大有助于珊瑚礁监测和管理工具的标准化。
Recruitment is a fundamental process that influences coral population dynamics as well as reef community structure. To date, coral recruitment success rates are poorly quantified because survey methods are labor-intensive and require manual interpretation. Thus, they are prone to human errors and have low repeatability - a gap we aim to bridge in this research. Since both corals and their symbiotic algae contain fluorescent pigments (chlorophyll and fluorescent proteins), we used the non-invasive Fluorescence Imaging System (FluorIS) and developed a methodology to acquire daytime fluorescent photographs and identify coral recruits in them. We tested our method by monitoring twenty random quadrats at two sites in the Gulf of Aqaba, Israel. The quadrats were surveyed once a month for eight months in order to track the settlement, mortality and survival rates of new coral recruits. We demonstrate daytime imaging using our method and identification of coral recruits as small as 1 mm in diameter, in a 20 x 20 cm quadrat. Our results show that this photographic method reduces surveyor errors and improves precision. The surveys revealed that on average, there are ~2 new coral recruit settlements (<2cm) for a quadrat (40cm2) per month and that 83% of them survive the first month. Our study suggests a relative stability in the Gulf of Aqaba coral population during the survey period. The ability to survey recruits during the day using low-cost, easy-to-use photographic equipment has the potential to contribute significantly to the standardization of coral reef monitoring and management tools, at a time when the world’s coral reefs are declining due to local and global stressors.
影响因子：
3.7
作者：
Alieva NO;Konzen KA;Field SF;Meleshkevitch EA;Hunt ME;Beltran-Ramirez V;Miller DJ;Wiedenmann J;Salih A;Matz MV
通讯作者：
Matz MV