A two‑stage electrophoretic microfluidic device for nucleic acid collection and enrichment
A two‑stage electrophoretic microfluidic device for nucleic acid collection and enrichment
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一种用于核酸收集和富集的两级电泳微流控装置
DOI：
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发表时间：
2016
期刊：
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通讯作者：
Yu-Hwa Lo
中科院分区：
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作者：
Wen Qiao;Chinhua Wang;Zengqian Ding;XiaoXiao Wei;Junlan Song;Yu-Hwa Lo
样品的纯化和富集是芯片核酸检测和分析的一个重要而又耗时的步骤。本文介绍了一种在微流控中电泳DNA聚焦的方法，将核酸浓度提高了约2700倍。devices.to施加到五个单独电极上的电波形被设计成使得DNA以高速连续地移动到收集电极，同时使由于电水解引起的气泡的干扰最小化。在总长48 cm的螺旋槽中，以30 μl/min的流速将1 ml DNA样品纯化富集57次。然后将捕获的DNA释放并输送到第二微流控室，在那里收集DNA并浓缩49倍。因此，在约40分钟内，两级装置可以从1 ml样品体积中提取DNA并将其浓度富集2790倍。在处理通量和DNA收集效率之间存在折衷。当流速为1 μl/min时，DNA捕获效率达到99.7%，当流速为30 μl/min时，DNA捕获通量达到最大。作为一种平台技术，该can.be集成到生物传感和遗传分析分析中，用于DNA提取和预浓缩。
Sample purification and enrichment is an important.and usually time-consuming step for on-chip nucleic.acid detection and analysis. This paper presents an electrophoretic.DNA focusing method in microfluidic devices.to enrich nucleic acid concentration by around 2700-fold..The electrical waveforms applied to five individual electrodes.are such designed that DNAs move successively to.the collection electrodes at high speed, while the interferences.from bubbles due to electrohydrolysis are minimized..In a spiral channel with a total length of 48 cm, 1 ml DNA.sample is purified and enriched by 57 times at a flow rate.of 30 μl/min at first. The captured DNAs are then released.and transported to the second microfluidic chamber where.DNAs are collected and concentrated by 49 times. Thus, in.about 40 min, the two-stage device can extract DNAs from.1 ml sample volume and enrich its concentration by 2790-.fold. A trade-off exists between the process throughput and.the DNA collection efficiency. A DNA capture efficiency.of 99.7 % is reached when the flow rate is 1 μl/min, and themaximum DNA capture throughput is achieved at a flow.rate of 30 μl/min. As a platform technology, the device can.be integrated into bio-sensing and genetic analysis assays.for DNA extraction and pre-concentration.