Carrier-dependency of cellular differentiation induced by bone morphogenetic protein in ectopic sites
Carrier-dependency of cellular differentiation induced by bone morphogenetic protein in ectopic sites
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DOI：
10.1016/s0901-5027(98)80071-4
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发表时间：
1998-10-01
影响因子：
2.4
通讯作者：
Nagai, N
中科院分区：
文献类型：
--
作者：
Murata, M;Inoue, M;Nagai, N
将部分纯化的骨形态发生蛋白（BMP）分别以多孔羟基磷灰石颗粒（PPHAP）和不溶性骨基质颗粒（IBM）为载体，植入大鼠皮下，观察BMP/PPHAP和BMP/IBM的成骨过程。BMP/PPHAP系统中的成骨主要是直接通过骨形成，类似于膜内成骨，而BMP/IBM系统中的成骨主要是软骨内成骨。BMP/PPHAP和BMP/IBM之间观察到的差异表明载体的结构和性质在骨诱导过程中的重要性。研究结果表明，骨和软骨分化不仅是由调节因子（BMP），但也通过其与载体的相互作用，BMP诱导的细胞分化是依赖于来自载体的微环境。
Partially purified bone morphogenetic protein (BMP) was delivered into two different types of carriers, porous particles of hydroxyapatite (PPHAP) and particles of insoluble bone matrix (IBM), and the ossification process was examined after subcutaneous implantation of the BMP/PPHAP and BMP/IBM in rats. The ossification in the BMP/PPHAP system was predominantly direct through bone formation similar to intramembranous ossification, whereas in the BMP/IBM system it was predominantly endochondral. The differences observed between the BMP/PPHAP and the BMP/IBM indicate the importance of the structure and nature of the carrier in the process of bone induction. The findings suggest that bone and cartilage differentiation is controlled not only by the regulation factor (BMP), but also by its interaction with the carrier, and that the BMP-induced cell differentiation is dependent upon the microenvironment derived from the carrier.