Comparison between fluxes of potassium and of chloride in astrocytes in primary cultures
Comparison between fluxes of potassium and of chloride in astrocytes in primary cultures
复制标题
原代培养物中星形胶质细胞钾和氯通量的比较
DOI：
10.1016/0006-8993(83)90940-x
复制
发表时间：
1983
期刊：
影响因子：
2.9
通讯作者：
L. Hertz
中科院分区：
文献类型：
--
作者：
W. Walz;L. Hertz
Transport processes operating in astrocytes were examined by measuring unidirectional fluxes of42K and36Cl in primary cultures of mouse astrocytes, at steady-state with respect to ion composition. The total K+uptake rate was 2025 nmol·mg−1protein·min−1. This rate was not influenced by furosemide (2 mM), an inhibitor of Cl−uptake and K+-K+exchange, or acetazolamide (0.1 mM), a carbonic anhydrase inhibitor. Ouabain (1 mM) inhibited the uptake rate by 541 nmol·mg−1·min−1. The equilibrated K+content was determined to be 696 nmol·mg−1. The rate constant for efflux was 2.76 min−1. This equals an efflux rate of 1921 nmol·mg−1·min−1, i.e. a similar value as the influx. Furosemide and ouabain did not inhibit the efflux. The eequilibrated Cl−contents was found to be 167 nmol·mg−1and it decreased in furosemide-treated cells to 68.1 nmol·mg−1. The total Cl−uptake was 35 nmol.mg−1and it was inhibited by furosemide or bumetanide by 27 nmol·mg−1·min−1. The means resting membrane potential was found to be—77.4 mV. From these data we conclude: (1) that the K+uptake rates are high, as can be expected from estimates based on literature data for K+conductance in mammalian glial cells in situ; and (2) that the cells possess a very low relative Cl−permeability.
人和豚鼠的神经胶质膜电位及其与 [K ]o 的关系。
影响因子：
4.1
作者：
Picker,S;Pieper,CF;Goldring,S
通讯作者：
Goldring,S