Monte-Carlo-based model for the extraction of intrinsic fluorescence from turbid media
Monte-Carlo-based model for the extraction of intrinsic fluorescence from turbid media
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DOI：
10.1117/1.2907161
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发表时间：
2008-03-01
影响因子：
3.5
通讯作者：
Ramanujam, Nirmala
中科院分区：
文献类型：
--
作者：
Palmer, Gregory M.;Ramanujam, Nirmala
开发了一种基于蒙特-卡罗的荧光模型，该模型能够提取组织的固有荧光特性，其独立于组织的吸收和散射特性。该模型是灵活的，在其适用性不同的照明收集几何形状，也是有效的广泛的光学特性，在紫外可见光谱的组织的代表。这在各种生物医学应用中是潜在有用的，包括癌症诊断和监测对治疗的生理反应。使用由血红蛋白（吸收体）、聚苯乙烯球（散射体）和呋喃-2（fluorophore）组成的phantomy验证该模型。结果表明，该模型能够恢复组织模型的固有荧光光谱，并恢复模型内呋喃的固有荧光强度的平均误差小于10%。(C)2008年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。
A Monte-Carlo-based model of fluorescence is developed that is capable of extracting the intrinsic fluorescence properties of tissue, which are independent of the absorption and scattering properties of tissue. This model is flexible in its applicability to different illumination-collection geometries and is also valid for a wide range of optical properties, representative of tissue in the UV-visible spectrum. This is potentially useful in a variety of biomedical applications, including cancer diagnostics and monitoring the physiological response to therapy. The model is validated using phantoms composed of hemoglobin (absorber), polystyrene spheres (scatterer), and furan-2 (fluorophore). It is found that this model is able to retrieve the intrinsic fluorescence spectra of the tissue phantoms and recover the intrinsic fluorescence intensity of furan within the phantoms to within a mean error of less than 10%. (C) 2008 Society of Photo-Optical Instrumentation Engineers.