Two natriuretic substances in extracts of urine from normal man when salt-depleted and salt-loaded.
Two natriuretic substances in extracts of urine from normal man when salt-depleted and salt-loaded.
复制标题
正常人在低盐和高盐情况下的尿液提取物中存在两种利尿钠物质。
DOI：
10.1038/ki.1976.124
复制
发表时间：
1976
影响因子：
19.6
通讯作者：
H. D. de Wardener
中科院分区：
文献类型：
--
作者：
E. Clarkson;S. M. Raw;H. D. de Wardener
当细胞外液体积扩大时，尿钠排泄量随之增加，部分原因是由于某些循环利钠物质浓度的变化。两种在凝胶层析上具有不同特性的利钠物质已经被不同的工作者分别鉴定出来。用31例正常人尿液提取液对正常清醒负重大鼠进行这两种利钠物质的检测。发现两个利钠组分。其中较大的在G50 Sephadex上制备，较小的在G25 Sephadex上制备。较大的物质产生的尿钠形成缓慢，持续两小时。较小的物质产生的尿钠在前20分钟最大，在接下来的40分钟内迅速下降，并在随后的60分钟内再次上升。从冻干尿液中提取的利钠活性被高浓度的氯化钠所降低。当受试者的尿液中含盐时，这两种物质的尿钠活性都比缺盐时更强。缺盐受试者的尿液中含有大量的利钠物质。
When the extra-cellular fluid volume is expanded, the subsequent rise in urinary sodium excretion that occurs is due in part to a change in the concentration of some circulating natriuretic substance. Two natriuretic substances with different characteristics on Sephadex chromatography have been previously been identified separately by different workers. Extracts prepared from the urine of 31 normal subjects were tested for these two natriuretic materials in the normal conscious water-loaded rat. Two natriuretic fractions were found. The larger of the two was prepared on G50 Sephadex, and the smaller on G25 Sephadex. The natriuresis produced by the larger material was slow to develop and persisted for two hours. The natriuresis produced by the smaller material was maximal in the first 20 min, declined rapidly within the next 40 min, and tended to rise again during the subsequent 60 min. The amount of natriuretic activity that could be extracted from the freeze-dried urine was diminished by high concentrations of sodium chloride. The natriuretic activity of both materials was greater in the urine of the subjects when they were salt-loaded than when they were salt-depleted. The urine of salt-depleted subjects contained significant amounts of natriuretic material.