"Non-Naked" Gold with Glucose Oxidase-Like Activity: A Nanozyme for Tandem Catalysis
"Non-Naked" Gold with Glucose Oxidase-Like Activity: A Nanozyme for Tandem Catalysis
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具有葡萄糖氧化酶样活性的“非裸露”金：用于串联催化的纳米酶
作者：
Zhang Haijiao;Liang Xin;Han Lei;Li Feng
已有广泛报道，没有保护剂的“裸”金纳米颗粒（Au NPs）具有葡萄糖氧化酶（GOx）样活性，并且使用保护剂可以抑制GOx样活性。在这里，通过使用蛋白质作为保护剂来合成具有GOx样活性的“非裸”Au NP。虽然“裸”Au纳米颗粒具有过氧化物酶样活性和GOx样活性，但两种活性的最佳pH范围明显不同。幸运的是，合成的“非裸”Au NPs在相同pH下显示出双重酶样活性。因此，“非裸”Au NPs可以被描述为“串联纳米酶”。作为另一个好处，蛋白质保护剂的参与可以稳定GOx样活性并使Au NPs可修饰。即使Au NP与氧化石墨烯（GO）连接，GOx样活性仍然没有改变。此外，将Au NPs‐GO纳米复合材料应用于一锅法非酶葡萄糖比色检测。“非裸”金不仅拓宽了串联纳米酶的种类，而且有利于纳米酶的功能化，在免疫分析、生物传感器和医疗等方面具有广阔的应用前景。
It has been widely reported that “naked” gold nanoparticles (Au NPs) without protectors have glucose oxidase (GOx)‐like activity, and the use of protectors can inhibit the GOx‐like activity. Here, “non‐naked” Au NPs with GOx‐like activity are synthesized by using protein as protector. Although “naked” Au NPs have peroxidase‐like activity and GOx‐like activity, the optimal pH ranges of the both activities are obviously different. Fortunately, as‐synthesized “non‐naked” Au NPs show the dual enzyme‐like activities at the same pH. So, “non‐naked” Au NPs can be described as “tandem nanozyme.” As another bonus, the participation of protein protector can stabilize the GOx‐like activity and make Au NPs modifiable. Even though Au NPs are connected with graphene oxide (GO), the GOx‐like activity is still not changed. Further, Au NPs‐GO nanocomposites are applied on the one‐pot nonenzymatic glucose colorimetric detection. The “non‐naked” gold not only broadens the species of tandem nanozymes, but also facilitates the functionalization of nanozymes, which is promising for immunoassay, biosensor, and medical treatment.