A rapid bioassay to determine stabilities of anticancer agents under conditions of the clonogenic assay.
A rapid bioassay to determine stabilities of anticancer agents under conditions of the clonogenic assay.
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一种快速生物测定，用于确定克隆形成测定条件下抗癌药物的稳定性。
DOI：
10.1007/bf02621226
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Kern,DH
中科院分区：
文献类型：
--
作者：
Hildebrand-Zanki,SU;Kern,DH
我们建立了一种快速的生物测定方法，用于在克隆试验中测定体外药物的稳定性。11种标准抗癌药物放线菌素D、阿霉素、博莱霉素、顺铂、达卡巴肼、5-氟尿嘧啶、马法兰、丝裂霉素C、1，3-二(2-氯乙基)-1-亚硝脲、长春花碱和长春新碱，以及四种研究药物氮杂环丁基苯醌(182986)、二氨基环丁烷-二羧基铂(241240)、二羟基蒽二酮盐酸盐(DHAD)(301739)和1-(2-chloroethyl)-3-(2，6-dioxo-3-piperidyl-1-nitrosourea)(NSC 95466)的体外半衰期在−40°C和−196°C条件下，BCNU和AZQ在−40℃下的t1/2分别为4.7d和2.5d。所有其他药物在−40°C和−196°C，t1/2>；6wk条件下均稳定。在37℃测定条件下，放线菌素D、博莱霉素、达卡巴肼、5-氟尿嘧啶、丝裂霉素C、长春花碱、长春新碱、长春新碱、阿霉素、顺铂、马法兰、BCNU、PCNU的t1/2分别为94、72、29、18.5、1.8、1、0.5h。
We developed a rapid bioassay to determine in vitro drug stabilities in the clonogenic assay. The in vitro half-lives of 11 standard antitumor agents, actinomycin D, Adriamycin, bleomycin, cis-Platinum, dacarbazine, 5-fluorouracil, melphalan, mitomycin C, 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), vinblastine, and vincristine, and four investigational drugs, Aziridinylbenzoquinone (AZQ) (NSC 182986), diamminecyclobutane-dicarboxylatoplatinum (CBDCA) (NSC 241240), Dihydroxyanthracenedione dihydrochloride (DHAD) (NSC 301739), and 1-(2-chloroethyl)-3-(2,6-dioxo-3-piperidyl-1-nitrosourea) (PCNU) (NSC 95466), were determined under conditions of the clonogenic assay as well as under storage conditions at −40° and−196°C. BCNU and AZQ at−40°C had t1/2 of 4.7 d and 2.5 d, respectively. All other drugs were stable at −40° and −196°C with t1/2>6 wk. Under assay conditions at 37°C, actinomycin D, bleomycin, dacarbazine, 5-fluorouracil, mitomycin C, vinblastine, vincristine, and DHAD were stable, with t1/2>14 d. CBDCA, AZQ, Adriamycin, cis-Platinum, melphalan, BCNU, and PCNU had t1/2 of 94,72,29,18.5,1.8,1, and 0.5 h, respectively.