SQUID MAJOR LENS POLYPEPTIDES ARE HOMOLOGOUS TO GLUTATHIONE S-TRANSFERASES SUBUNITS
SQUID MAJOR LENS POLYPEPTIDES ARE HOMOLOGOUS TO GLUTATHIONE S-TRANSFERASES SUBUNITS
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DOI：
10.1038/336086a0
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发表时间：
1988-11-03
期刊：
影响因子：
64.8
通讯作者：
ZINOVIEVA, RD
中科院分区：
文献类型：
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作者：
TOMAREV, SI;ZINOVIEVA, RD
头足类动物和脊椎动物的眼睛晶状体的进化相对较晚，并且通过独立的途径1。它们为蛋白质水平上的趋同进化研究提供了良好的实验模型。一项提议是，在进化过程中，预先存在的蛋白质被招募为结构性眼晶状体蛋白质2。这已在脊椎动物眼晶状体结构蛋白（即 crystal-lins3-9）中得到证实，该蛋白已被深入研究 10,11。尽管有关头足类眼睛 Ienses12-14 的信息有限，但有人认为谷胱甘肽 S 转移酶 (GST) 可能是鱿鱼大晶状体蛋白的进化祖先。最近，鱿鱼大晶状体蛋白的 N 端序列与大鼠 GST 的 Ya 亚基有 55% 同源性。在这里，我们证明鱿鱼大晶状体多肽是由至少三个成员的基因家族编码的。我们表征了与这些基因相对应的两个 cDNA，并表明它们本身可能是 GST 亚基，或者与它们共享一个进化祖先。
The eye lenses of cephalopods and vertebrates evolved relatively recently and by independent routes1. They provide a good experimental model for the study of convergent evolution at the protein level. One proposal is that pre-existing proteins were recruited as structural eye lens proteins during evolution2. This has been confirmed for the vertebrate eye lens structural proteins, or crystal-lins3–9, which have been intensively studied10,11. Despite the limited information about cephalopod eye Ienses12–14, it has been suggested that glutathionine S-transf erases (GSTs) are a possible evolutionary ancestor of the squid major lens proteins. Recently, the N-terminal sequence of the squid major lens protein was shown3to be 55% homologous with that of the Ya subunit of the rat GST. Here, we demonstrate that the squid major lens polypeptides are encoded by a gene family of at least three members. We characterize two cDNAs corresponding to these genes and show they probably either are GST subunits themselves, or share an evolutionary ancestor with them.