Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan)
Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan)
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DOI：
10.1111/j.1462-2920.2010.02338.x
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发表时间：
2011-08-01
影响因子：
5.1
通讯作者：
Kamagata, Yoichi
中科院分区：
文献类型：
--
作者：
Mayumi, Daisuke;Mochimaru, Hanako;Kamagata, Yoichi
通过对采出水和原油进行孵育，结合放射性示踪剂实验和分子生物学分析，研究了高温油藏中产甲烷菌群和产甲烷途径。在模拟原位环境（55℃，5 MPa）的高温和加压条件下，不加任何底物修饰进行孵育。在孵育过程中甲烷和醋酸盐浓度的变化表明醋酸盐产生甲烷的化学计量学。使用[(14)C]-碳酸氢盐测量的氢营养化产甲烷率是使用[2-(14)C]-醋酸盐测量的醋酸裂解产甲烷率的42-68倍，这表明在环境中，甲烷的产生主要是通过乙酸合营养化氧化与氢营养化产甲烷相结合。对生产水的16S rRNA基因序列分析显示，细菌群落以热产菌属为主，被称为嗜热合养醋酸氧化菌，而古细菌群落以嗜热氢养产甲烷菌属为主，属于嗜热甲烷菌属。此外，群体特异性实时PCR分析显示，产甲烷杆菌目加氢产甲烷菌的16S rRNA基因拷贝数与古细菌的16S rRNA基因拷贝数几乎相同。研究表明，醋酸合营养氧化是高温油藏产甲烷的主要途径。
The methanogenic communities and pathways in a high-temperature petroleum reservoir were investigated through incubations of the production water and crude oil, combined with radiotracer experiments and molecular biological analyses. The incubations were conducted without any substrate amendment and under high-temperature and pressurized conditions that mimicked the in situ environment (55 degrees C, 5 MPa). Changes in methane and acetate concentrations during the incubations indicated stoichiometric production of methane from acetate. Rates of hydrogenotrophic methanogenesis measured using [(14)C]-bicarbonate were 42-68 times those of acetoclastic methanogenesis measured using [2-(14)C]-acetate, implying the dominance of methane production by syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the environment. 16S rRNA gene sequence analyses of the incubated production water showed bacterial communities dominated by the genus Thermacetogenium, known as a thermophilic syntrophic acetate-oxidizing bacterium, and archaeal communities dominated by thermophilic hydrogenotrophic methanogens belonging to the genus Methanothermobacter. Furthermore, group-specific real-time PCR assays revealed that 16S rRNA gene copy numbers of the hydrogenotrophic methanogens affiliated with the order Methanobacteriales were almost identical to those of archaeal 16S rRNA genes. This study demonstrates that syntrophic acetate oxidation is the main methanogenic pathway in a high-temperature petroleum reservoir.