THE ROLE OF DNA DOUBLE-STRAND BREAK REPAIR THROUGH NON-HOMOLOGOUS END JOINING IN THE DOSE-RATE EFFECT IN TERMS OF CLONOGENIC ABILITY
THE ROLE OF DNA DOUBLE-STRAND BREAK REPAIR THROUGH NON-HOMOLOGOUS END JOINING IN THE DOSE-RATE EFFECT IN TERMS OF CLONOGENIC ABILITY
复制标题
通过非同源末端连接进行 DNA 双链断裂修复在克隆形成能力方面的剂量率效应中的作用
DOI：
10.1093/rpd/ncac030
复制
发表时间：
2022
影响因子：
1
通讯作者：
Matsumoto Yoshihisa
中科院分区：
文献类型：
--
作者：
Tsuchiya Hisayo;Shimada Mikio;Tsukada Kaima;Meng Qingmei;Kobayashi Junya;Matsumoto Yoshihisa
人们普遍认为，给定剂量的电离辐射，特别是X射线和伽马射线等低线性能量传递辐射的生物效应，随着剂量率的降低而变小。这种现象被称为剂量率效应(DRE)，被认为是由于辐射过程中亚致死性损伤的修复，但DRE的确切机制仍有待阐明。我们最近发现，在缺乏Ku的啮齿动物细胞中，克隆细胞存活方面的Dre减少甚至逆转，Ku是通过非同源末端连接(NHEJ)修复DNA双链断裂(DSB)的关键因素之一。在这里，我们回顾和讨论NHEJ在DRE中的参与，这在放射防护和癌症治疗中具有潜在的意义。
It is generally and widely accepted that the biological effects of a given dose of ionizing radiation, especially those of low linear energy transfer radiations like X-ray and gamma ray, become smaller as the dose rate becomes lower. This phenomenon, known as ‘dose-rate effect (DRE),’ is considered due to the repair of sublethal damage during irradiation but the precise mechanisms for DRE have remained to be clarified. We recently showed that DRE in terms of clonogenic cell survival is diminished or even inversed in rodent cells lacking Ku, which is one of the essential factors in the repair of DNA double-strand breaks (DSBs) through non-homologous end joining (NHEJ). Here we review and discuss the involvement of NHEJ in DRE, which has potential implications in radiological protection and cancer therapeutics.