DIETARY ETHANOL STIMULATES THE ACTIVITY OF PHOSPHATIDYLCHOLINE-SPECIFIC PHOSPHOLIPASE-D AND THE FORMATION OF PHOSPHATIDYLETHANOL IN DROSOPHILA-MELANOGASTER LARVAE
DIETARY ETHANOL STIMULATES THE ACTIVITY OF PHOSPHATIDYLCHOLINE-SPECIFIC PHOSPHOLIPASE-D AND THE FORMATION OF PHOSPHATIDYLETHANOL IN DROSOPHILA-MELANOGASTER LARVAE
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DOI：
10.1016/0965-1748(93)90049-x
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发表时间：
1993-09-01
影响因子：
3.8
通讯作者：
GEER, BW
中科院分区：
文献类型：
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作者：
MILLER, RR;YATES, JW;GEER, BW
当在第三龄果蝇幼虫的饮食中施用短链伯醇时，短链伯醇会降低磷脂酰胆碱 (PC) 水平。乙醇诱导的幼虫 PC 减少可能部分归因于 PC 特异性磷脂酶 D（PC 特异性 PLD，EC 3.1.4.4）活性的增加。 PC特异性PLD不仅能水解PC，而且显然还能催化磷脂酰乙醇的形成。 200 mM 乙醇、甲醇、异丙醇、正丁醇和正丙醇也可刺激 PC 特异性 PLD 活性。体外研究表明，亚微摩尔浓度的 Ca2+ 和 GTP-gammaS 可以增强果蝇 PC 特异性 PLD 活性。利用[C-14]溶血棕榈酰磷脂酰胆碱的体内研究表明，膳食乙醇促进标记物流入三酰基甘油、1,2二酰基甘油和脂肪酸乙酯，而PC中的标记物减少。
When administered in the diet to third instar Drosophila melanogaster larvae, short chain primary alcohols reduce phosphatidylcholine (PC) levels. The ethanol-induced reductions in larval PC may be in part due to an increase in the activity of PC-specific phospholipase D (PC-specific PLD, EC 3.1.4.4). PC-specific PLD not only hydrolyzes PC, but it also apparently catalyzes the formation of phosphatidylethanol. PC-specific PLD activity was also stimulated by 200 mM ethanol, methanol, isopropanol, n-butanol, and n-propanol. In vitro studies indicated that Drosophila PC-specific PLD activities were enhanced by submicromolar concentrations of Ca2+ and by GTP-gammaS. In vivo studies utilizing [C-14]lyso-palmitoyl phosphatidylcholine indicated that dietary ethanol promoted the flux of label into triacylglycerol, 1, 2 diacylglycerol, and fatty acid ethyl esters, while the label in PC decreased.