Coexistence of bacterial sulfide oxidizers, sulfate reducers, and spirochetes in a gutless worm (Oligochaeta) from the Peru margin
Coexistence of bacterial sulfide oxidizers, sulfate reducers, and spirochetes in a gutless worm (Oligochaeta) from the Peru margin
复制标题
DOI：
10.1128/aem.71.3.1553-1561.2005
复制
发表时间：
2005-03-01
影响因子：
4.4
通讯作者：
Dubilier, N
中科院分区：
文献类型：
--
作者：
Blazejak, A;Erséus, C;Dubilier, N
粗齿Olavius crassitunicatus是一种小型的共生蠕虫，在东太平洋的缺氧沉积物中大量存在。利用比较16 S rRNA序列分析和荧光原位杂交，我们研究了两个地理上相距遥远的O.在秘鲁边缘（水深约300米）的crassitunicatus种群（相隔385公里）。五种不同的细菌共生类型共同发生在所有标本从这两个网站：两个成员的γ-变形菌（γ 1和2共生体），两个成员的三角洲变形菌（三角洲I和2共生体），和一个螺旋体。第六种属于三角洲变形菌（三角洲3共生体）的细菌类型被发现在两个宿主种群中只有一个。三个O。Crassitunicatus细菌共生菌型与其它无肠寡毛类的共生菌密切相关，Gamma 1型与Olavius algarvensis、Olavius loisae和Inanidrilus leukodermatus的硫化物氧化共生菌密切相关，Delta 1型与O . algarvensis的螺旋体，该螺旋体与O.洛伊萨与此相反，伽马2型和三角洲2和3型属于新的谱系，与其他细菌共生体无关。在海洋无脊椎动物中，多种细菌共生类型共存于一个宿主中，这种共生遗传多样性又高度特异性和稳定性的关系以前从未被描述过。
Olavius crassitunicatus is a small symbiont-bearing worm that occurs at high abundance in oxygen-deficient sediments in the East Pacific Ocean. Using comparative 16S rRNA sequence analysis and fluorescence in situ hybridization, we examined the diversity and phylogeny of bacterial symbionts in two geographically distant O. crassitunicatus populations (separated by 385 km) on the Peru margin (water depth, similar to 300 m). Five distinct bacterial phylotypes co-occurred in all specimens from both sites: two members of the gamma-Proteobacteria (Gamma 1 and 2 symbionts), two members of the delta-Proteobacteria (Delta I and 2 symbionts), and one spirochete. A sixth phylotype belonging to the delta-Proteobacteria (Delta 3 symbiont) was found in only one of the two host populations. Three of the O. crassitunicatus bacterial phylotypes are closely related to symbionts of other gutless oligochaete species; the Gamma 1 phylotype is closely related to sulfide-oxidizing symbionts of Olavius algarvensis, Olavius loisae, and Inanidrilus leukodermatus, the Delta 1 phylotype is closely related to sulfate-reducing symbionts of O . algarvensis, and the spirochete is closely related to spirochetal symbionts of O. loisae. In contrast, the Gamma 2 phylotype and the Delta 2 and 3 phylotypes belong to novel lineages that are not related to other bacterial symbionts. Such a phylogenetically diverse yet highly specific and stable association in which multiple bacterial phylotypes coexist within a single host has not been described previously for marine invertebrates.