Microfluidic pressure in paper (μPiP): rapid prototyping and low-cost liquid handling for on-chip diagnostics
Microfluidic pressure in paper (μPiP): rapid prototyping and low-cost liquid handling for on-chip diagnostics
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DOI：
10.1039/d1an01676h
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发表时间：
2021-12-30
期刊：
影响因子：
4.2
通讯作者：
Gagnon, Zachary R.
中科院分区：
文献类型：
--
作者：
Islam, Md Nazibul;Yost, Jarad W.;Gagnon, Zachary R.
纸基微流控最初是为液体吸气被动驱动的超低成本诊断而开发的。然而，在用纸利用外部施加的压力梯度在内部引导多孔微流控流动方面，仍有巨大的潜力尚未开发。在这里，我们提出了一种利用外压制造和利用低成本聚合物叠层纸基微流控器件的新技术。通过这种技术制造的设备称为纸中微流体压力(Mu PIP)，能够保持压力梯度，用于精确的液体处理和操纵应用，类似于传统的微流控明渠设计，但在流体直接通过多孔纸结构的情况下。MU PIP设备既可以快速制作原型，也可以按商业规模制造和部署，只需最少的时间、设备和培训要求。我们对多孔纸基微流控通道中的连续压力驱动流动进行了分析，并展示了该方法在各种不同液体处理应用中的广泛适用性，包括测量红细胞变形性和进行连续自由流动DNA电泳。这一新平台为执行微流控操作提供了一种经济实惠的方法，既可用于学术原型，也可用于大规模商业设备生产。
Paper-based microfluidics was initially developed for use in ultra-low-cost diagnostics powered passively by liquid wicking. However, there is significant untapped potential in using paper to internally guide porous microfluidic flows using externally applied pressure gradients. Here, we present a new technique for fabricating and utilizing low-cost polymer-laminated paper-based microfluidic devices using external pressure. Known as microfluidic pressure in paper (mu PiP), devices fabricated by this technique are capable of sustaining a pressure gradient for use in precise liquid handling and manipulation applications similar to conventional microfluidic open-channel designs, but instead where fluid is driven directly through the porous paper structure. mu PiP devices can be both rapidly prototyped or scalably manufactured and deployed at commercial scale with minimal time, equipment, and training requirements. We present an analysis of continuous pressure-driven flow in porous paper-based microfluidic channels and demonstrate broad applicability of this method in performing a variety of different liquid handling applications, including measuring red blood cell deformability and performing continuous free-flow DNA electrophoresis. This new platform offers a budget-friendly method for performing microfluidic operations for both academic prototyping and large-scale commercial device production.