Acute phase phospholipids related to the cardiolipin of mitochondria in the sera of patients with chronic fatigue syndrome (CFS), chronic Ciguatera fish poisoning (CCFP), and other diseases attributed to chemicals, Gulf War, and marine toxins
Acute phase phospholipids related to the cardiolipin of mitochondria in the sera of patients with chronic fatigue syndrome (CFS), chronic Ciguatera fish poisoning (CCFP), and other diseases attributed to chemicals, Gulf War, and marine toxins
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DOI：
10.1002/jcla.20217
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发表时间：
2008-01-01
影响因子：
2.7
通讯作者：
Yabusaki, Kenichi
中科院分区：
文献类型：
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作者：
Hokama, Yoshitsugi;Empey-Campora, Cara;Yabusaki, Kenichi
本研究采用改良的膜免疫珠试验（MIA）检测了328份CFS血清，其中包括17份CCFP、8份海湾战争退伍军人（GWV）、24份前列腺癌（PC）和52份正常血清。用纯化的单克隆抗体-CTX作MIA检测328例CFS患者血清，91.2%的抗体滴度≥ 1：40。17份CCFP血清样本中的76%和8份GWV血清样本中的100%的滴度也>= 1：40。52份正常人血清中92.3%滴度≤ 1：20，4份滴度≥ 1：40。此外，通过商业ELISA程序检查了41份血清的抗心磷脂（aCL），其中87.8%显示IgM、IgM+伊加或IgM +IgG aCL抗体。这些结果表明，大多数IgM抗体单独在血清样品。此外，还对41份血清样本进行了aCL检查，其中37份显示aCL阳性，占所检查的三种疾病类别（CFS、CCFP和GWV）的90.2%。28例患者（CFS（18例）、CCFP（5例）和GWV（5例））的抗线粒体-M2自身抗体（aM-M2）检查均为阴性。用来自患者血清的抗原CTX、CIFS“急性期脂质”、市售心磷脂（CL）和1，2-二棕榈酰-sn-甘油3-[磷酸-L-丝氨酸]（PS）以及抗体MAb-CTX和aCL进行的抑制分析显示CL和CTX中的磷脂与抗体MAb-CTX和CFS-aCL在抗原性上不可区分。初步化学分析表明，脂质是与线粒体CL相关的磷脂。我们将其命名为“急性期脂质”，与炎症条件下的“急性期蛋白”（C反应蛋白（CRP）和血清淀粉样蛋白A（SAA））相当。
This study examined 328 CFS sera in a study with 17 CCFP, 8 Gulf War Veterans (GWV), 24 Prostate Cancer (PC), and 52 normal sera in the modified Membrane Immunobead Assay (MIA) procedure for CTX. Three hundred and twenty-eight CFS patients' sera were examined by the modified MIA with purified MAb-CTX and 91.2% gave a titre >= 1:40. 76% of the 17 CCFP sera samples and 100% of the 8 GWV sera samples also had a titre >= 1:40. 92.3% of 52 normal sera showed titres of 1:20 or less, while 4 gave titres of >= 1:40. In addition, 41 sera were examined for Anti-Cardiolipin (aCL) by a commercial ELISA procedure with 87.8% demonstrating IgM, IgM+IgA, or lgM+IgG aCL antibodies. These results showed mostly the IgM aCL antibody alone in the sera samples. In addition, 41 serum samples were examined for aCL, with 37 showing positive for aCL, representing 90.2% positive for the three disease categories examined: CFS, CCFP and GWV. Examination for antiMitochondrial-M2 autoantibody (aM-M2) in 28 patients (CFS (18), CCFP (5), and GWV (5)) was negative for aM-M2. Inhibition analysis with antigens, CTX, CIFS "Acute Phase Lipids", commercial Cardiolipin (CL) and 1,2-Dipalmitoyl-sn-Glycero3-[Phospho-L-Serine] (PS) and antibodies, MAb-CTX and aCL from patients' serum show that the phospholipids in CL and CTX are antigenically indistinguishable with antibodies MAb-CTX and CFS-aCL. Preliminary chemical analyses have shown the lipids to be phospholipids associated with CL of the mitochondria. We designate this "Acute Phase Lipid" comparable to "Acute Phase Proteins" (C-reactive protein (CRP) and Serum Amyloid A (SAA)) in inflammatory conditions.