The Toxicity of Silver Nanoparticles Depends on Their Uptake by Cells and Thus on Their Surface Chemistry
The Toxicity of Silver Nanoparticles Depends on Their Uptake by Cells and Thus on Their Surface Chemistry
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DOI：
10.1002/ppsc.201300215
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发表时间：
2013-12-01
影响因子：
2.7
通讯作者：
Parak, Wolfgang J.
中科院分区：
文献类型：
--
作者：
Caballero-Diaz, Encarnacion;Pfeiffer, Christian;Parak, Wolfgang J.
制备了一组三种类型的银纳米颗粒（Ag NP），它们具有相同的银核，但表面化学性质不同。Ag核用巯基十一烷酸（MUA）或用聚合物壳[聚（异丁烯-alt-马来酸酐）（PMA）]稳定。为了减少细胞摄取，聚合物包覆的Ag NP另外用聚乙二醇（PEG）修饰。对NP的腐蚀（氧化）进行定量，并研究它们的胶体稳定性。MUA-涂覆的NP具有比PMA-涂覆的NP低得多的胶体稳定性，并且大部分聚集。所有Ag NP在酸性环境中腐蚀更快，因此更多的Ag（I）离子在内体/溶酶体隔室内释放。与MUA涂层相比，PMA涂层不会减少Ag（I）离子的浸出。PEG化降低NP细胞摄取以及毒性。PMA包被的NP与MUA包被的NP相比毒性降低。所有研究的银纳米颗粒都比游离Ag（I）离子无毒。总而言之，Ag NPs的细胞毒性与其被细胞摄取和聚集行为相关。
A set of three types ofsilver nanoparticles (Ag NPs) are prepared, whichhave the same Ag cores, but different surface chemistry. Ag cores are stabilized with mercaptoundecanoic acid (MUA) or with a polymer shell [poly(isobutylene-alt-maleic anhydride) (PMA)]. In order to reduce cellular uptake, the polymer-coated Ag NPs are additionally modified with polyethylene glycol (PEG). Corrosion (oxidation) of the NPs is quantified and their colloidal stability is investigated. MUA-coated NPs have a much lower colloidal stability than PMA-coated NPs and are largely agglomerated. All Ag NPs corrode faster in an acidic environment and thus more Ag(I) ions are released inside endosomal/lysosomal compartments. PMA coating does not reduce leaching of Ag(I) ions compared with MUA coating. PEGylation reduces NP cellular uptake and also the toxicity. PMA-coated NPshavereduced toxicity compared with MUA-coated NPs. All studied Ag NPs wereless toxic than free Ag(I) ions. All in all, the cytotoxicity of Ag NPs is correlated on their uptake by cells and agglomeration behavior.