Hydrogenimonas thermophila gen. nov., sp. nov., a novel thermophilic, hydrogen-oxidizing chemolithoautotroph within the epsilon-Proteobacteria, isolated from a black smoker in a Central Indian Ridge hydrothermal field.
Hydrogenimonas thermophila gen. nov., sp. nov., a novel thermophilic, hydrogen-oxidizing chemolithoautotroph within the epsilon-Proteobacteria, isolated from a black smoker in a Central Indian Ridge hydrothermal field.
复制标题
DOI：
10.1099/ijs.0.02787-0
复制
发表时间：
2004
影响因子：
2.8
通讯作者：
K. Takai;K. Nealson;K. Horikoshi
中科院分区：
文献类型：
--
作者：
K. Takai;K. Nealson;K. Horikoshi
一种新的嗜热细菌，菌株EP1-55-1%T，从位于印度洋中印度洋脊的Kairei Field的过热深海热液喷口的原位定植系统中分离出来。这些细胞是高度运动的杆状细胞，每个细胞都有一个单极鞭毛。生长在35 ~ 65℃（最适温度55℃，加倍时间70 min）和pH 4.9 ~ 7.2（最适pH 5.9）之间。该分离物是一种以氢分子为唯一能源，二氧化碳为唯一碳源的微生物-厌氧化石自养生物。分子氧、硝酸盐或单质硫（S0）可以作为电子受体支持生长。基因组DNA中G+C含量为34.6% mol%。基于16S rDNA序列的系统发育分析表明，菌株EP1-55-1%T是第一个在未培养的epsilon-Proteobacteria A群中具有分类特征的菌株；提出以菌株EP1-55-1%T （=JCM 11971T=ATCC BAA-737T）为型菌株，命名为嗜热氢单胞菌gen. nov., sp. 11 .。
A novel thermophilic bacterium, strain EP1-55-1%T, was isolated from an in-situ colonization system deployed in a superheated, deep-sea, hydrothermal vent emission at the Kairei Field on the Central Indian Ridge in the Indian Ocean. The cells were highly motile rods, each possessing a single polar flagellum. Growth was observed between 35 and 65 degrees C (optimum temperature, 55 degrees C; 70 min doubling time) and between pH 4.9 and 7.2 (optimum, pH 5.9). The isolate was a microaerobic-to-anaerobic chemolithoautotroph capable of using molecular hydrogen as the sole energy source and carbon dioxide as the sole carbon source. Molecular oxygen, nitrate or elemental sulfur (S0) could serve as electron acceptors to support growth. The G+C content of the genomic DNA was 34.6 mol%. Phylogenetic analysis based on 16S rDNA sequences indicated that strain EP1-55-1%T represents the first strain for which taxonomic properties have been characterized within the previously uncultivated phylogroup classified as belonging to the uncultivated epsilon-Proteobacteria group A; the name Hydrogenimonas thermophila gen. nov., sp. nov. is proposed, with strain EP1-55-1%T (=JCM 11971T=ATCC BAA-737T) as the type strain.