Neuroproteomics of the synapse and drug addiction
Neuroproteomics of the synapse and drug addiction
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DOI：
10.1124/jpet.105.091520
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发表时间：
2006-08-01
影响因子：
3.5
通讯作者：
Devi, Lakshmi A.
中科院分区：
文献类型：
--
作者：
Abul-Husn, Noura S.;Devi, Lakshmi A.
脑组织的蛋白质组学分析正在成为神经科学研究的一个组成部分。特别是，突触在神经传递和可塑性中的重要作用已经带来了广泛的努力来确定其蛋白质成分。最近的研究结合了亚细胞分离和蛋白质组学技术来鉴定与突触不同成分相关的蛋白质。因此，一个连贯的突触蛋白质组图正在迅速出现，及时审查这些数据是必要的。在这篇综述的第一部分，神经蛋白质组学技术已经被用来分析突触蛋白质组进行了描述。然后，我们总结了最近几项哺乳动物突触蛋白质组学分析的结果，并讨论了它们在突触蛋白谱上的异同。该研究领域的重要进展包括利用蛋白质组学分析突触功能和药物对突触蛋白的影响。本文概述了突触蛋白磷酸化状态的蛋白质组学分析以及神经蛋白质组学技术在药物成瘾研究中的最新应用。最后，我们讨论了比较药物成瘾的蛋白质组学研究所面临的挑战，以及该领域未来的发展方向，以进一步了解突触功能和药物成瘾的分子机制。
Proteomic analyses of brain tissues are becoming an integral component of neuroscientific research. In particular, the essential role of the synapse in neurotransmission and plasticity has brought about extensive efforts to identify its protein constituents. Recent studies have used a combination of subcellular fractionation and proteomic techniques to identify proteins associated with different components of the synapse. Thus, a coherent map of the synapse proteome is rapidly emerging, and a timely review of these data is warranted. In the first part of this review, neuroproteomic techniques that have been used to analyze the synapse proteome are described. We then summarize the results from several recent proteomic analyses of mammalian synapses and discuss the similarities and differences in their profiling of synaptic proteins. Important advances in this field of research include the use of proteomics to analyze synaptic function and drug effects on synaptic proteins. This article presents an overview of proteomic analyses of the phosphorylation states of synaptic proteins and recent applications of neuroproteomic techniques to the study of drug addiction. Finally, we discuss the challenges in comparing proteomic studies of drug addiction and the future directions of this field in furthering our understanding of the molecular mechanisms underlying synaptic function and drug addiction.