Influence of the wettability of different titanium surface topographies on initial cellular behavior
Influence of the wettability of different titanium surface topographies on initial cellular behavior
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DOI：
10.4012/dmj.2017-334
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发表时间：
2018-08-01
影响因子：
2.5
通讯作者：
Koyano, Kiyoshi
中科院分区：
文献类型：
--
作者：
Nishimura, Tomoko;Ogino, Yoichiro;Koyano, Kiyoshi
本研究探讨了不同表面形貌的时间依赖性润湿性对初始细胞行为的影响。制备了具有光滑形貌（SM）和三种粗糙形貌（喷砂（SA）、微形貌（M）和纳米形貌（N））的钛圆片。如先前的研究所示，在所有表面中观察到表面润湿性的时间依赖性变化。在SM表面上，疏水性改变影响细胞扩散和RhoA（Rho家族的小GTdR蛋白）的活性，而在粗糙表面上除了粘附细胞的数量外没有观察到任何改变。血清吸附可以通过疏水性改变恢复这些功能退化。这些研究结果表明，表面形貌是一个更有力的调节器在初始细胞行为，如细胞扩散和RhoA激活比表面润湿性。
This study examined the influence of the time-dependent wettability of different surface topographies on initial cellular behavior. Titanium disks with smooth topography (SM) and three kinds of rough topography (sandblasted (SA), microtopography (M) and nanotopography (N)) were prepared. Time-dependent changes in surface wettability were observed in all surfaces as shown in previous studies. On SM surfaces, hydrophobic alteration influenced cell spreading and the activity of RhoA (a small GTPase protein of the Rho family), while no alterations were observed on rough surfaces except for the number of adherent cells. Serum adsorption could recover these functional deteriorations by hydrophobic alteration. These findings suggest that surface topography is a more potent regulator in initial cellular behaviors such as cell spreading and RhoA activation than surface wettability.