MECHANISMS FOR HEMOLYSIS BY ULTRASONIC CAVITATION IN THE ROTATING EXPOSURE SYSTEM
MECHANISMS FOR HEMOLYSIS BY ULTRASONIC CAVITATION IN THE ROTATING EXPOSURE SYSTEM
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DOI：
10.1016/0301-5629(91)90124-f
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发表时间：
1991-01-01
影响因子：
2.9
通讯作者：
WILLIAMS, AR
中科院分区：
文献类型：
--
作者：
MILLER, DL;THOMAS, RM;WILLIAMS, AR
旋转室提供了一个有用的系统，用于增强空化生物效应，以便更容易地研究它们。将具有透声窗的管垂直于其轴以72 rpm旋转。1.61 MHz超声波束在42 ms脉冲串中打开和关闭，以暴露腔室，同时与波束对准。0.5%犬红细胞悬液的溶血被用作该旋转暴露系统的有效性的量度。使用透析膜、琼脂塞、塑料密封膜或蜡膜作为窗口，在高于2-2.8 W/cm 2的表观阈值（最高达16 W/cm 2）的暴露强度下，观察到基本上100%溶解的结果无差异。将管长度从1.4 cm改变为4 cm，或将其分成三个0.5 cm的段对结果几乎没有影响，但短的0.5 cm腔室降低了有效性。在IOMPa下对悬浮液填充室加压以减少空化核的群体减少了溶血。分别加压悬浮液或室产生难以区分的，较小的减少溶血。结果导致假设溶血主要发生在培养基的主体中（而不是表面上）。通过旋转再循环到培养基中的气泡以10 m/s的估计速度向腔室后部移动，产生足以引起观察到的溶血的流体动力学应力。
The rotating chamber provides a useful system for enhancing cavitation bioeffects so that they may be more easily studied. A tube with acoustically transparent windows was rotated at 72 rpm perpendicular to its axis. The 1.61 MHz ultrasound beam was switched on and off in 42 ms bursts to expose the chamber while it was aligned with the beam. The hemolysis of 0.5% suspensions of canine erythrocytes was used as a measure of the efficacy of this rotating exposure system. Use of dialysis membrane, agar plugs, plastic sealing film or wax film for the windows produced no differences in the results for exposure intensities above an apparent threshold of 2-2.8 W/cm2, up to 16 W/cm2, at which essentially 100% lysis was observed. Changing the tube length from 1.4 cm to 4 cm, or partitioning it into three 0.5 cm segments had little influence on the results, but a short 0.5 cm chamber had reduced efficacy. Pressurizing the suspension filled chamber at 10 MPa to reduce the population of cavitation nuclei reduced the hemolysis. Separately pressurizing the suspension or the chamber produced indistinguishable, smaller reductions in hemolysis. The results lead to the hypothesis that the hemolysis occurs primarily in the bulk of the medium (rather than on surfaces). Bubbles recycled by rotation into the medium move toward the back of the chamber at estimated speeds of 10 m/s, generating hydrodynamic stresses sufficient to cause the observed hemolysis.