Analysis of hydrophilic metabolites by high-performance liquid chromatography-mass spectrometry using a silica hydride-based stationary phase
Analysis of hydrophilic metabolites by high-performance liquid chromatography-mass spectrometry using a silica hydride-based stationary phase
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DOI：
10.1016/j.chroma.2008.07.077
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发表时间：
2008-09-12
影响因子：
4.1
通讯作者：
Sana, Theodore R.
中科院分区：
文献类型：
--
作者：
Pesek, Joseph J.;Matyska, Maria T.;Sana, Theodore R.
采用一种新型的硅氢化硅固定相，以水正相(ANP)为标准，评价了三类代谢物的保留行为。考察了改变柱温、在流动相和样品溶剂中加入不同的添加剂对氨基酸、碳水化合物和小分子有机酸保留行为的影响。梯度模式结果表明，对于这些化合物类，保留时间的重复性非常稳定。在15摄氏度和30摄氏度下，对于超过50次的氨基酸混合物注射，获得了良好的RSD值，变异小于1%。展示了分离19种非衍生氨基酸标准、有机酸和碳水化合物的能力，以及这种材料分离尿液等复杂液体中极性代谢物的潜力。(C)2008爱思唯尔B.V.保留所有权利。
A novel silica hydride-based stationary phase was used to evaluate the retention behavior in the aqueous normal-phase (ANP) mode of standards representing three classes of metabolites. The effects on retention behavior of amino acids, carbohydrates and small organic acids were examined by altering the column temperature, and by adding different additives to both the mobile phase and sample solvent. Gradient mode results revealed the repeatability of retention times to be very stable for these compound classes. At both 15 and 30 degrees C, excellent RSD values were obtained with less than 1% variation for over 50 injections of an amino acid mixture. The ability to separate the 19 nonderivatized amino acid standards, organic acids and carbohydrates was demonstrated as well as the potential for this material to separate polar metabolites in complex fluids such as urine. (c) 2008 Elsevier B.V. All rights reserved.