Comparative studies on glycoproteins expressing polylactosamine-type N-glycans in cancer cells
Comparative studies on glycoproteins expressing polylactosamine-type N-glycans in cancer cells
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DOI：
10.1016/j.jpba.2012.06.035
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发表时间：
2012-11-01
影响因子：
3.4
通讯作者：
Kakehi, Kazuaki
中科院分区：
文献类型：
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作者：
Mitsui, Yosuke;Yamada, Keita;Kakehi, Kazuaki
在我们之前的系列报告中，我们表明一些癌细胞系特异性表达聚乳糖胺型N-聚糖，并且此类聚糖通常被岩藻糖和硫酸盐残基修饰。为了确认表达这些聚糖的蛋白质，通过用胰蛋白酶消化整个蛋白质级分获得的糖肽混合物被聚乳糖胺特异性凝集素、曼陀罗凝集素（DSA）捕获。用 N-糖酰胺酶 F 消化后捕获的糖肽中的肽和聚糖通过 HPLC 和 MS 技术进行了广泛分析。我们发现，在所有检查的癌细胞中，一些糖蛋白（例如 CD107a 和 CD107b）通常含有聚乳糖胺型聚糖。但具有这些聚糖的整合素-α 5 (CD49e) 和癌胚抗原相关细胞粘附分子 5 (CD66e) 分别在 U937（人 T 淋巴瘤）和 MKN45（人胃癌）细胞中特异发现。这些数据清楚地表明，附着在特定蛋白质上的特定聚糖将成为特定肿瘤的高精度标记物。 (C) 2012 Elsevier B.V. 保留所有权利。
In the series of our previous reports, we showed that some cancer cell lines specifically express polylactosamine-type N-glycans and such glycans were often modified with fucose and sulfate residues. To confirm the proteins expressing these glycans, glycopeptide mixture obtained by digestion of whole protein fractions with trypsin was captured by a polylactosamine-specific lectin, Datura strasmonium agglutinin (DSA). And the peptides and glycans of the captured glycopeptides after digestion with N-glycoamidase F were extensively analyzed by HPLC and MS techniques. We found that some glycoproteins such as CD107a and CD107b commonly contained polylactosamine-type glycans in all the examined cancer cells. But integrin-alpha 5 (CD49e) and carcinoembryonic antigen-related cell adhesion molecule 5 (CD66e) having these glycans were specifically found in U937 (human T-lymphoma) and MKN45 (human gastric cancer) cells, respectively. These data clearly indicate that specific glycans attached to specific proteins will be promising markers for specific tumors with high accuracy. (C) 2012 Elsevier B.V. All rights reserved.