AFFINITY OF 4 IMMOBILIZED ERYTHRINA LECTINS TOWARD VARIOUS N-LINKED GLYCOPEPTIDES AND RELATED OLIGOSACCHARIDES
AFFINITY OF 4 IMMOBILIZED ERYTHRINA LECTINS TOWARD VARIOUS N-LINKED GLYCOPEPTIDES AND RELATED OLIGOSACCHARIDES
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DOI：
10.1016/s0008-6215(00)90355-0
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发表时间：
1986-08-15
影响因子：
3.1
通讯作者：
SHARON, N
中科院分区：
文献类型：
--
作者：
DEBRAY, H;MONTREUIL, J;SHARON, N
研究了N-乙酰乳糖胺型寡糖和糖肽在四种不同刺桐凝集素固定化琼脂糖凝胶柱上的行为。四种凝集素的糖结合特异性非常相似，并且针对未掩蔽的N-乙酰乳糖胺序列，四种凝集素之间的主要差异是凝集素与给定聚糖的相互作用的相对强度。在半乳糖的O-3或O-6位用唾液酸残基取代N-乙酰乳糖胺序列完全消除了凝集素对β-内酰胺的亲和力。一个或几个α- Fuc-（1 →）3)-GlcNAc基团降低或完全抑制糖肽和刺桐凝集素之间的相互作用。β的取代-甘露糖残基通过另外的平分β-（1）4）与未取代的结构相比，-N-乙酰葡糖胺残基降低了凝集素对这些结构的亲和力。令人惊讶的是，凝集素对所测试的寡糖的亲和力高于对相应的糖肽的亲和力。我们的研究结果表明，经过仔细的校准与定义明确的寡糖和糖肽，固定化刺桐凝集素-琼脂糖柱提供了有价值的工具，N-乙酰乳糖胺含寡糖和糖肽的分馏。
The behavior of N-acetyllactosamine-type oligosaccharides and glycopeptides on columns of four different Erythrina agglutinins immobolized on Sepharose was examined. The sugar-binding specificity of the four lectins is very similar and is directed toward unmasked N-acetyllactosamine sequences, the main difference between the four lectins being the relative strength of interaction of the lectins with a given glycan. Substitution of the N-acetyllactosamine sequences by sialic acid residues, either at O-3 or O-6 of galactose completely abolishes the affinity of the lectins for the saccharides. The presence of one or several .alpha.-Fuc-(1 .fwdarw. 3)-GlcNAc groups decreases or completely inhibits the interaction between the glycopeptides and the Erythrina lectins. Substitution of the .beta.-mannose residue by an additional bisecting .beta.-(1 .fwdarw. 4)-N-acetylglucosamine residue decreases the affinity of the lectins for these structures as compared to the unsubstituted ones. Surprisingly, the affinity of the lectins for the oligosaccharides tested is higher than for the corresponding glycopeptides. Our findings show that, after careful calibration with well-defined oligosaccharides and glycopeptides, the immobilized Erythrina agglutinin-Sepharose columns provide valuable tools for the fractionation of N-acetyllactosamine-containing oligosaccharides and glycopeptides.