Plasmonic Optical Fiber Based Continuous in-Vivo Glucose Monitoring for ICU/CCU Setup
Plasmonic Optical Fiber Based Continuous in-Vivo Glucose Monitoring for ICU/CCU Setup
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DOI：
10.1109/tnb.2023.3303345
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发表时间：
2023-08
影响因子：
3.9
通讯作者：
Souvik Kundu;Shawana Tabassum;Ritwesh A. Kumar;E. D. Abel;Fellow Ieee Ratnesh Kumar;Ratnesh Kumar
中科院分区：
文献类型：
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作者：
Souvik Kundu;Shawana Tabassum;Ritwesh A. Kumar;E. D. Abel;Fellow Ieee Ratnesh Kumar;Ratnesh Kumar
本文报告了一种传感器体系结构，用于直接连续监测血液中的生物标志物，特别是对于需要危重护理和快速生物标志物测量的ICU/CCU患者。该传感器基于一个简单的光纤，可以通过导管插入血流，其中金纳米颗粒连接在其远端作为等离子体材料，通过表面等离子体激元的激发对目标生物分子(在我们的应用中是葡萄糖)进行高灵敏的光化学传感。为了特异性，纳米颗粒被特定的受体酶功能化，从而实现基于局部表面等离子共振(LSPR)的靶向生物传感。此外，在拟议的体系结构中引入了微透析探头，这有助于在较长时间内连续监测，而不会污染传感器表面，使整个血液中存在的细胞和血液碎片，导致相对于现有的最先进的连续监测设备可以在血液中进行直接测量，提高了灵敏度和检测极限。为了建立这一概念验证，我们测试了传感器设备，以监测体内的血糖，包括动物模型，其中连续监测直接在活体大鼠的循环中进行。该传感器对葡萄糖的灵敏度为0.0354 AU/mg.dl−1，检测下限为50.89 mg/dl。
This paper reports a sensor architecture for continuous monitoring of biomarkers directly in the blood, especially for ICU/CCU patients requiring critical care and rapid biomarker measurement. The sensor is based on a simple optical fiber that can be inserted through a catheter into the bloodstream, wherein gold nanoparticles are attached at its far distal end as a plasmonic material for highly sensitive opto-chemical sensing of target biomolecules (glucose in our application) via the excitation of surface plasmon polaritons. For specificity, the nanoparticles are functionalized with a specific receptor enzyme that enables the localized surface plasmon resonance (LSPR)-based targeted bio-sensing. Further, a micro dialysis probe is introduced in the proposed architecture, which facilitates continuous monitoring for an extended period without fouling the sensor surface with cells and blood debris present in whole blood, leading to prolonged enhanced sensitivity and limit of detection, relative to existing state-of-the-art continuous monitoring devices that can conduct direct measurements in blood. To establish this proof-of-concept, we tested the sensor device to monitor glucose in-vivo involving an animal model, where continuous monitoring was done directly in the circulation of living rats. The sensor’s sensitivity to glucose was found to be 0.0354 a.u./mg.dl−1 with a detection limit of 50.89 mg/dl.