Effect of 1,25-dihydroxyvitamin D3 on cytosolic calcium in dispersed parathyroid cells.
Effect of 1,25-dihydroxyvitamin D3 on cytosolic calcium in dispersed parathyroid cells.
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1,25-二羟基维生素 D3 对分散的甲状旁腺细胞中胞质钙的影响。
DOI：
10.1038/ki.1988.76
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发表时间：
1988
影响因子：
19.6
通讯作者：
Slatopolsky,E
中科院分区：
文献类型：
--
作者：
Sugimoto,T;Ritter,C;Ried,I;Morrissey,J;Slatopolsky,E
1，25-二羟基维生素D3对分散的甲状旁腺细胞胞浆钙的影响我们使用荧光染料indo-1检测了1，25-二羟维生素D3（1，25（OH）2D 3）对分散的牛甲状旁腺细胞胞浆钙（[Ca]i）的影响。加入10− 8 M1，25（OH）2D 3在10分钟内使[Ca] i增加23.4 ± 2.7%。在加入1，25-（OH）_2D_3的两分钟内，[Ca] i显著增加。1，25-（OH）2D 3以剂量依赖性方式增加[Ca] i，并且仅在10− 10 M时发生。10− 7 M25-（OH）D3和10− 7 M24，25-（OH）2D 3均未引起[Ca]i显著增加。用EGTA螯合细胞外钙可阻断1，25-（OH）2D 3诱导的[Ca]i增加，表明[Ca]i的增加主要来自细胞外钙。10− 5 Mverapamil和10− 4 Mdiltiazem都不能阻断1，25-（OH）2D 3诱导的[Ca]i增加。目前的数据表明，1，25-（OH）_2D_3可能改变膜的钙渗透性，而不依赖于对维拉帕米或地尔硫卓敏感的电压依赖性钙通道。1，25-（OH）2D 3的快速效应提出了其机制不依赖于基因组激活的可能性，可能归因于与甲状旁腺细胞质膜组分的直接相互作用。
Effect of 1,25-dihydroxy vitamin D3on cytosolic calcium in dispersed parathyroid cells. We examined the effect of 1,25-dihydroxyvitamin D3(1,25(OH)2D3) on cytosolic calcium ([Ca]i) of dispersed bovine parathyroid cells, using the fluorescent dye indo-1. The addition of 10−8M1,25(OH)2D3caused an increase in [Ca]iby 23.4 ± 2.7% over a 10 minute period. There was a significant increase in [Ca]iwithin two minutes of the addition of 1,25-(OH)2D3. 1,25-(OH)2D3increased [Ca]iin a dose-dependent manner and this occurred with as little as 10−10M. Neither 10−7M25-(OH)D3nor 10−7M24, 25-(OH)2D3caused a significant increase in [Ca]i. Chelation of extracellular calcium with EGTA blocked the 1,25-(OH)2D3-induced increase in [Ca]i, suggesting that the increase was mainly from extracellular calcium. Neither 10−5Mverapamil nor 10−4Mdiltiazem blocked the 1,25-(OH)2D3-induced increase in [Ca]i. The present data suggest that 1,25-(OH)2D3might modify membrane permeability to calcium independent of voltage-dependent calcium channels sensitive to verapamil or diltiazem. The rapid effect of 1,25-(OH)2D3raises the possibility that its mechanism is independent of genome activation, perhaps attributable to direct interaction with components of the parathyroid cell plasma membrane.