A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures
A universal method for the preparation of covalent protein-DNA conjugates for use in creating protein nanostructures
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DOI：
10.1002/anie.200701942
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发表时间：
2007-01-01
影响因子：
16.6
通讯作者：
Distefano, Mark D.
中科院分区：
文献类型：
--
作者：
Duckworth, Benjamin P.;Chen, Yan;Distefano, Mark D.
蛋白质图案化：用叠氮化物标签选择性标记的蛋白质与炔官能化DNA反应。这些蛋白质-DNA缀合物用于产生DNA组装的蛋白质纳米结构。这种方法应有利于制备一系列具有有用的结构和功能特征的蛋白质-DNA杂交结构。
(Figure Presented) Protein patterning: A protein, selectively labeled with an azide tag, was reacted with alkyne-functionalized DNA. These protein-DNA conjugates were used to create a DNA-assembled protein nanostructure. This method should facilitate the preparation of a range of protein-DNA hybrid structures with useful structural and functional features.