CHONDROGENESIS OF MESENCHYMAL STEM CELLS IN A NOVEL HYALURONATE-COLLAGEN-TRICALCIUM PHOSPHATE SCAFFOLDS FOR KNEE REPAIR
CHONDROGENESIS OF MESENCHYMAL STEM CELLS IN A NOVEL HYALURONATE-COLLAGEN-TRICALCIUM PHOSPHATE SCAFFOLDS FOR KNEE REPAIR
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DOI：
10.22203/ecm.v031a06
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发表时间：
2016-01-01
影响因子：
3.1
通讯作者：
Liao, W. M.
中科院分区：
文献类型：
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作者：
Meng, F. G.;Zhang, Z. Q.;Liao, W. M.
支架有望在诱导骨髓间充质干细胞(MSCs)向软骨组织再生的过程中发挥关键作用。在这里，我们报道了一种新型的磷酸三钙-胶原-透明质酸钙(TcP-Col-HA)支架的研制，该支架可以作为干细胞载体来诱导软骨生成和促进软骨修复，并研究了不同含量的TcP、Col和HA支架的软骨诱导特性。比较了两种不同比例(50：50和25：75)的Tcp-Col-HA支架和不同Tcp/Col比例(50：50和25：75)的支架在体内外诱导兔骨髓间充质干细胞(RMSCs)再生的能力。通过硫酸糖胺多聚糖定量、II型胶原免疫组织化学和qRT-PCR检测软骨分化程度。通过压缩试验评价材料的机械强度。结果表明，在促进RMSC软骨形成方面，TcP-Col-HA支架的促进作用强于Tcp-Col支架；对于后者，较低的Tcp/Col比例(25：75)的支架促进RMSC软骨化的效果更好。在裸鼠异位移植模型中也得到了类似的结果。在临界大小的兔骨软骨缺损修复模型中，种植在TCP-Col-HA支架上的rMSCs表现出比TCP-Col组更好的软骨再生和与周围组织的整合，其中25：75的软骨修复效率高于50：50的软骨修复效率。这些结果表明，添加HA和不同的TcP/Col比例可以影响支架的软骨诱导能力，提示Tcp-Col-HA支架可以作为一种有效的软骨再生细胞载体。
Scaffolds are expected to play a key role in the induction of chondrogenesis of mesenchymal stem cells (MSCs) for cartilage tissue regeneration. Here, we report the development of a novel tricalcium phosphate-collagen-hyaluronate (TCP-COL-HA) scaffold that can function as a stem cell carrier to induce chondrogenesis and promote cartilage repair, and the investigation of chondroinductive properties of scaffolds containing varying amounts of TCP, COL and HA. TCP-COL-HA scaffolds, as well as TCP-COL scaffolds at two different TCP/COL ratios (50: 50 and 25: 75), were evaluated for their ability to induce cartilage regeneration from rabbit mesenchymal stem cells (rMSCs) in vitro and in vivo. Chondrogenic differentiation was evaluated by sulphated glycosaminoglycan quantification, collagen type II immunohistochemistry, and qRT-PCR. Mechanical strength was evaluated by the compression test. The results showed that the TCP-COL-HA scaffolds enhanced rMSC chondrogenesis to a greater degree than did the TCP-COL scaffolds; for the latter, the scaffold with the lower TCP/COL ratio (25: 75) was superior in terms of promoting rMSC chondrogenesis. Similar results were obtained in an ectopic implantation model in nude mice. In a critical-size rabbit osteochondral defect-repair model, rMSCs seeded on TCP-COL-HA scaffolds showed greater cartilage regeneration and integration into surrounding tissue than the TCP-COL groups, in which cartilage repair was more efficient at the 25: 75 than at the 50: 50 ratio. These results indicate that the addition of HA and different TCP/COL ratios can affect the chondroinductive capacity of scaffolds, and suggest that the TCP-COL-HA scaffold can serve as an effective cell carrier for cartilage regeneration.