Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity
Epstein-Barr Virus BBRF2 Is Required for Maximum Infectivity
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DOI：
10.3390/microorganisms7120705
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发表时间：
2019-12-01
期刊：
影响因子：
4.5
通讯作者：
Murata, Takayuki
中科院分区：
文献类型：
--
作者：
Al Masud, H. M. Abdullah;Yanagi, Yusuke;Murata, Takayuki
EB病毒（EBV）是γ疱疹病毒亚科的成员，其引起传染性单核细胞增多症和几种类型的癌症。BBRF 2是EBV的一个未表征的基因，在裂解期表达。为了评估其功能，使用细菌人工染色体（BAC）技术和CRISPR/Cas9系统制备BBRF 2敲除EBV。尽管病毒基因表达、DNA合成和子代分泌不受影响，但子代病毒的感染性显著降低。当单独表达时，BBRF 2蛋白定位于细胞核和细胞质，而相互作用的伴侣BSRF 1的共表达导致其重新定位于细胞质。有趣的是，BBRF 2的共表达保护BSRF 1免受蛋白酶体/泛素依赖性降解。因此，BBRF 2与BSRF 1一起增强病毒感染性。
Epstein-Barr virus (EBV) is a member of the gammaherpesvirinae, which causes infectious mononucleosis and several types of cancer. BBRF2 is an uncharacterized gene of EBV and is expressed during the lytic phase. To evaluate its function, BBRF2-knockout EBV was prepared using bacterial artificial chromosome (BAC) technology and the CRISPR/Cas9 system. Although viral gene expression, DNA synthesis, and progeny secretion were not affected, the infectivity of progeny viruses was significantly reduced by the disruption of BBRF2. When expressed alone, BBRF2 protein localized to the nucleus and cytoplasm, while the coexpression of an interacting partner, BSRF1, resulted in its relocalization to the cytoplasm. Interestingly, the coexpression of BBRF2 protected BSRF1 from proteasome/ubiquitin-dependent degradation. Therefore, BBRF2, together with BSRF1, augments viral infectivity.