A novel murine oncornavirus with dual eco- and xenotropic properties.
A novel murine oncornavirus with dual eco- and xenotropic properties.
复制标题
一种具有生态和异嗜双重特性的新型小鼠肿瘤病毒。
DOI：
--
复制
发表时间：
1975
影响因子：
11.1
通讯作者：
D. Bolognesi
中科院分区：
文献类型：
--
作者：
P. Fischinger;S. Nomura;D. Bolognesi
用莫洛尼白血病病毒（MLV-IC）的克隆分离株感染瑞士小鼠 3T3FL 细胞，产生了由至少三种不同的小鼠辅助癌病毒组成的病毒后代。通过几次连续的终端稀释分离在适当的细胞中纯化每个实体并生长至高滴度。除了生态性 MLV-IC 之外，还有一种纯异嗜性病毒和第三种具有生态性和异嗜性病毒特性的新型病毒。纯化的异源病毒具有广泛的宿主范围，仅限于小鼠细胞内，并且与其他异源分离株一样被正常小鼠血清灭活。具有混合特性（HIX）的纯化病毒可以感染多种哺乳动物细胞，其中包括 N 和 B 小鼠细胞。 HIX 对小鼠和猫指示细胞进行了相同滴度的单次滴定。通过病毒感染前干扰、病毒糖蛋白干扰以及特异性抗血清中和来检查 HIX 的包膜特性。在病毒外壳上发现了异嗜性和MLV-IC型亲嗜性决定簇。对 HIX 和异种病毒的起源进行了详细研究。原始 MLV-IC 库存含有低滴度的 HIX 型病毒，但没有检测到纯异种病毒。用来自 MLV-IC 病毒库的亲嗜性病毒的单个感染单位感染小鼠细胞有时可能会产生 HIX 型病毒。 HIX型病毒在异源大鼠细胞中传代一次后，在受感染的小鼠或猫细胞中进行长期传代。几个月后，HIX 型病毒从一些小鼠和猫细胞系统中消失。讨论了 HIX 可能的杂交性质和新出现的异种病毒的起源。
Infection of Swiss mouse 3T3FL cells with a clonal isolate of Moloney leukemia virus (MLV-IC) resulted in virus progeny composed of at least three different murine helper oncornaviruses. Each entity was purified in appropriate cells by several sequential terminal dilution isolations and was grouwn to high titers. Besides ecotropic MLV-IC there was a pure xenotropic virus and a third novel virus with properties of both eco- and xenotropic viruses. The purified xenotropic virus had a wide host range, was restricted in mouse cells, and was inactivated by normal mouse sera like other xenotropic isolates. The purified virus with hybrid properties (HIX) could infect a wide range of mammalian cells, which included both N and B mouse cells. HIX gave single-hit titrations with equal titers on both mouse and cat indicator cells. Envelope properties of HIX were examined by virus preinfection interference, by interference involving viral glycoprotein, and by neutralization with specific antisera. Both xenotropic and MLV-IC type ecotropic determinants were found on the virus coat. The origins of HIX and the xenotropic virus were investigated in detail. The original MLV-IC stock had HIX type virus in low titer but no detectable pure xenotropic virus. Infection of mouse cells with a single infectious unit of the ecotropic virus from the MLV-IC virus stocks could at times give rise to HIX type virus. HIX type virus, passed once through heterologous rat cells, was subjected to long-term passage either in infected mouse or cat cells. After several months HIX type virus disappeared from some mouse and cat cell systems. The possible hybrid nature of HIX and the origins of newly appearing xenotropic viruses are discussed.