Functional Analysis of MicroRNAs in Skeletal Muscle
Functional Analysis of MicroRNAs in Skeletal Muscle
复制标题
骨骼肌中 MicroRNA 的功能分析
DOI：
10.1007/978-1-0716-3036-5_24
复制
发表时间：
2023
影响因子：
--
通讯作者：
Akimoto Takayuki
中科院分区：
文献类型：
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作者：
Oikawa Satoshi;Akimoto Takayuki
微小RNA（microRNAs，miRNAs）是一类在脊椎动物中高度保守的非编码小分子RNA，在多种生物学过程中发挥重要作用。miRNA通过加速mRNA的降解和/或通过抑制蛋白质翻译来微调基因表达。肌肉特异性miRNAs的鉴定扩展了我们对骨骼肌分子网络的认识。在这里，我们描述了通常用于分析骨骼肌中miRNA功能的方法。
MicroRNAs (miRNAs) are small non-coding RNAs that are highly conserved in vertebrates and play important roles in diverse biological processes. miRNAs function to fine-tune gene expression by accelerating the degradation of mRNA and/or by inhibiting protein translation. Identification of muscle-specific miRNAs has extended our knowledge of the molecular network in skeletal muscle. Here we describe methods that are commonly used to analyze the function of miRNAs in skeletal muscle.